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Chimerigen Laboratories



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The Experts for High Quality Fusion Proteins

Since many years Chimerigen Laboratories, LLC (Chimerigen) develops, manufactures and markets high quality and leading edge proteins for biomedical and immunology research. One of Chimerigen's specialty is the production of unique immunoglobulin based chimeric fusion proteins using advanced cellular and molecular biological techniques. These reagents are used successfully in basic and applied research. AdipoGen has now become a trusted and reliable marketing and sales partner for Chimerigen's product panel. Because of the high performance characteristics and quality the Chimerigen fusion proteins are widely recognized reagents and are cited in many scientific publications.

 

Non-lytic Ig-based Chimeric Fusion Cytokines with Long Circulating Half-life

The potential clinical application of cytokines to modulate immune responses is very high. Unfortunately, most cytokines have short circulating half-lives. Therefore, to facilitate the study of cytokine effects in vivo, a variety of non-lytic immunoglobulin-based chimeric cytokine fusion proteins have been created, in which a cytokine sequence had been genetically fused to the hinge, CH2 and CH3 regions of an immunoglobulin. 


These non-lytic fusion proteins possess both the biological functions of the cytokine moiety and a prolonged circulating half-life determined by the Fc domain. Mutations to the complement (C1q) and FcgR I binding sites of the IgGs Fc fragment render the fusion proteins incapable of antibody directed cytotoxicity (ADCC) and complement directed cytotoxicity (CDC). These fusion molecules also have the promise of being minimally to negligibly immunogenic since they are made entirely from elements derived from the species to be treated.

Structure of Fusion Proteins     
Figure:
General structure of mouse non-lytic fusion proteins.

Literature:
Localization of the binding site for the human high-affinity Fc receptor on IgG: A.R. Duncan, et al.; Nature 332, 563 (1988)
The binding site for Clq on IgG: A.R. Duncan & G. Winter; Nature 332, 738 (1988)
Administration of noncytolytic IL-10/Fc in murine models of lipopolysaccharide-induced septic shock and allogeneic islet transplantation: X.X. Zheng, et al.; J. Immunol. 154, 5590 (1995)


Chimerigen Laboratories, LLC and Adipogen International, Inc. provide all Chimerigen Products worldwide. Please contact us for more information.

For Product Inquiries: info@chimerigen.ch



Newly Released from Chimerigen Laboratories


PID Product Name         Size Source Purity Endotoxin
 CHI-MF-111T3  Tim-3 (mouse):Fc (human) (rec.)  100 µg  CHO cells  ≥98%
 <0.06EU/μg
 CHI-HF-220EPO  EPO (human):Fc (human) (rec.) (non-lytic) 50 µg  CHO cells ≥98%   <0.06EU/μg
 CHI-ZHF-21002S
 IL-2 Superkine (Fc)
 MultiPack
10 µg
3 x 10 µg
HEK293 cells  ≥95%
 <0.01EU/μg
 CHI-MF-11135  IL-35 (mouse):Fc (human) (rec.) 5 µg  CHO cells  ≥98%
 <0.06EU/μg



Selected High Quality Fusion Proteins from Chimerigen


Fc Fusion Proteins    Highlights

PID Product Name         Size Source Purity Endotoxin
CHI-HF-210A4                      CD152 [CTLA-4] (human):Fc (human) (rec.)                100 µg
     500 µg
       1 mg
    NS1 cells   ≥98%       <0.06EU/μg
CHI-MF-110A4 CD152 [CTLA-4] (mouse):Fc (mouse) (rec.) 100 µg
500 µg
1 mg
     NS1 cells ≥98% <0.06EU/μg
CHI-HF-21002 IL-2 (human):Fc (human) (rec.)
MultiPack
50 µg
        3 x 50 µg
CHO cells ≥98% <0.06EU/μg
CHI-HF-21006 IL-6 (human):Fc (human) (rec.)
MultiPack
50 µg
3 x 50 µg
CHO cells ≥98% <0.06EU/μg
CHI-HF-21015M IL-15 (mutant) (human):Fc (human) (rec.) 50 µg CHO cells ≥98% <0.06EU/μg
CHI-HF-21115MBI IL-15 (mutant) (human):Fc (mouse) (rec.) (Biotin) 1 Vial CHO cells ≥98% <0.06EU/μg
CHI-HF-210T1 Tim-1 (human):Fc (human) (rec.) 100 µg CHO cells ≥98% <0.06EU/μg
CHI-HF-210T3 Tim-3 (human):Fc (human) (rec.) 100 µg CHO cells ≥98% <0.06EU/μg
CHI-HF-210T4 Tim-4 (human):Fc (human) (rec.) 100 µg CHO cells ≥98% <0.06EU/μg
CHI-HF-210TGFBM TGFß1 (mutant) (human):Fc (human) (rec.) 100 µg CHO cells ≥98% <0.06EU/μg

Non-lytic Fc Fusion Proteins    Highlights

PID Product Name         Size Source Purity Endotoxin
CHI-MF-120A4              CD152 [CTLA-4] (mouse):Fc (mouse) (rec.) (non-lytic)
             100 µg
     500 µg
       1 mg
     NS1 cells       ≥98%       <0.06EU/μg
CHI-HF-22002
IL-2 (human):Fc (human) (rec.) (non-lytic)
MultiPack
50 μg
3 x 50 μg
NS1 cells ≥98% <0.06EU/μg
CHI-MF-12002
IL-2 (mouse):Fc (mouse) (rec.) (non-lytic)
MultiPack
10 μg
5 x 10 μg
NS1 cells ≥98% <0.06EU/μg
CHI-HF-22004
IL-4 (human):Fc (human) (rec.) (non-lytic)
MultiPack
10 μg
5 x 10 μg
NS1 cells ≥98% <0.06EU/μg
CHI-MF-12004
IL-4 (mouse):Fc (mouse) (rec.) (non-lytic)
MultiPack
10 μg
5 x 10 μg
NS1 cells ≥98% <0.06EU/μg
CHI-HF-22006
IL-6 (human):Fc (human) (rec.) (non-lytic)
MultiPack
50 μg
3 x 50 μg
CHO cells ≥98% <0.06EU/μg
CHI-MF-12006
IL-6 (mouse):Fc (mouse) (rec.) (non-lytic)
MultiPack
50 μg
3 x 50 μg
CHO cells ≥98% <0.06EU/μg
CHI-HF-22010
IL-10 (human):Fc (human) (rec.) (non-lytic)
MultiPack
10 μg
5 x 10 μg
NS1 cells ≥98% <0.06EU/μg
CHI-MF-12010
IL-10 (mouse):Fc (mouse) (rec.) (non-lytic)
MultiPack
10 μg
5 x 10 μg
NS1 cells ≥98% <0.06EU/μg



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More Information

  Product Flyer

Please contact us at info@chimerigen.ch to receive a print copy of the 
Chimerigen Fusion Protein Product Flyer 2014! 

Also available for download!










  Chimerigen Catalog 2014

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Please contact us at info@chimerigen.ch to receive a print copy of the 
Chimerigen Fusion Protein Product Flyer 2013! 

Also available for download!











  Chimerigen Catalog

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Please contact us at info@chimerigen.ch to receive a print copy of the 
Chimerigen Fusion Protein Product Supplement Flyer 2013!

Also available for download!











  Chimerigen Catalog Supplement 2013

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