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MultimericLigands™ - Higher Activity - Lower Endotoxin




AdipoGen® Multimeric Proteins are high activity constructs in which two trimeric TNFSF ligands are linked via the oligomeric collagen domain of ACRP30 [ACRP30headless] and therefore mimic the membrane-bound forms of the proteins.



The Largest Panel of Ultrapotent MultimericLigands™ 

Equivalent to MegaLigands!



Endogenous TNF superfamily ligands are either active as membrane-form (e.g. FasL, TRAIL, CD40L, OX40L) or are secreted and activated through oligomerization by the binding of proteoglycans at the surface of cells (e.g. APRIL).

The oligomerization is triggered

• by fusing the TNF superfamily ligands to the the collagen domain of the protein ACRP30 (which itself has no functional activity) to form a hexameric structure and therefore creating “Multimeric Proteins”, or

• by adding a cross-linking antibody called “TNF Ligands Enhancer” (Prod. No. AG-35B-0001).


  Multimeric replaces Mega



Multimeric Ligands Structure

MultiPacks and BULK for In Vivo



MultimericFasL™ [ MegaFasL]


FasL (human) (multimeric) (rec.)

AG-40B-0130-C010                                                10 μg
AG-40B-0130-3010          MultiPack                 3 x 10 μg

• MultimericFasL™ very effectively simulates the naturalmembrane-assisted aggregation of FasL in vivo.

Source: HEK 293 cells.

Sequence: Human FasL (aa 139-281) is fused at theN-terminus to mouse ACRP30headless(aa 18-111) and a FLAG®-tag.

Specificity: Binds to human and mouse Fas.

Biological Activity: Induces apoptosis of human JurkatT cells at a concentration of <1ng/ml.

Endotoxin Content: <0.02EU/μg purified protein (LAL test; Lonza).

Literature References:
 • Two adjacent trimeric Fas ligands are required for Fassignaling and formation of a death-inducing signaling complex: N. Holler, et al.;Mol. Cell. Biol. 23, 1428 (2003)
 • A Fas agonist induces high levels of apoptosisin haematological malignancies: P. Greaney, et al.; Leuk. Res. 30, 415 (2006)

 


MultimericFasL

Figure:
Oligomerisation of FasL (human) efficiently triggers Jurkat cell death.
Method: Jurkat cells were treated O/N with the indicated concentrations of FasL (human) (multimeric) (rec.) (AG-40B-0130), Fc (human):FasL (human) (rec.) (AG-40B-0132), FasL (human) (rec.) (AG-40B-0001) or FasL (human) (rec.) + Enhancer (AG-44B-0001) (2 fold-dilutions, first concentration of 1000ng/ml). Cell death was quantified using PMS/MTS. The oligomeric FasL recombinant proteins (FasL (human) (multimeric), Fc (human):FasL (human) and FasL (human) + Enhancer) kill Jurkat cells at IC50 <0.2ng/ml.



MultimericCD40L™


CD40L (human) (multimeric) (rec.)

AG-40B-0010-C010                                                10 μg
AG-40B-0010-3010          MultiPack                 3 x 10 μg

MultimericCD40L™ very effectively mimics the natural membrane-assisted aggregation of CD40L. It is the most potent alternative to activate CD40. No enhancer is required.

MultimericCD40L™ is a potent B cell activator.

Literature References:
 • IgG subclass switch capacity is low in switched and in IgM-only, but high in IgD+IgM+, post-germinal center (CD27+) human B cells: C. Werner-Favre, et al.; Eur. J. Immunol. 31, 243 (2001)

 
MultimericCD40L

Figure:
 CD40L (human) (multimeric) (rec.) (Prod. No. AG-40B-0010) does not need an enhancer to induce B cells activation.

CD40L (mouse) (multimeric) (rec.)
>> BULK available for in vivo studies!
AG-40B-0017-C010
AG-40B-0017-3010
10 μg
MultiPack           3 x 10 μg
CD40L (rat) (multimeric) (rec.)
>> BULK available for in vivo studies!
AG-40B-0088-C010
AG-40B-0088-3010
10 μg
MultiPack           3 x 10 μg


Full Panel of MultimericLigands


APRIL (human) (multimeric) (rec.) AG-40B-0017-C010
AG-40B-0017-3010
10 μg
MultiPack           3 x 10 μg
APRIL (human) (H98) (multimeric) (rec.) AG-40B-0088-C010
AG-40B-0088-3010
10 μg
MultiPack           3 x 10 μg
APRIL (mouse) (multimeric) (rec.) AG-40B-0089-C010
AG-40B-0089-3010
10 μg
MultiPack           3 x 10 μg
APRIL (mouse) (H98) (multimeric) (rec.) AG-40B-0035-C010
AG-40B-0035-3010
10 μg
MultiPack           3 x 10 μg
OX40L (mouse) (multimeric) (rec.) AG-40B-0029-C010
10 μg
TNF-α (human) (multimeric) (rec.)
>> Activates TNF-R1 and TNF-R2!
AG-40B-0019-C010
10 μg


For more information on all AdipoGen Multimeric Ligands please search this website.

For Product Inquiries: info@adipogen.com