AdipoGen Life Sciences

OX40L (mouse) (multimeric) (rec.)

CHF 220.00

In stock

AG-40B-0029-C01010 µgCHF 220.00

Equivalent to MegaLigands

More Information
Product Details
Synonyms	MultimericOX40L™; ACRP30headless:OX40L; ACRP30headless:CD252; ACRP30headless:CD134L
Product Type	Protein
Properties
Source/Host	HEK 293 cells
Sequence	The extracellular domain of mouse OX40L (aa 52-199) is fused at the N-terminus to mouse ACRP30headless (aa 18-111) and a FLAG^®-tag.
Crossreactivity	Human Mouse
Specificity	Binds to human and mouse OX40 [CD134].
Biological Activity	Activates T cell proliferation.
MW	~40kDa (SDS-PAGE)
Purity	≥90% (SDS-PAGE)
Endotoxin Content	<0.01EU/μg purified protein (LAL test; Lonza).
Concentration	0.1mg/ml after reconstitution.
Reconstitution	Reconstitute with 100μl sterile water.
Formulation	Lyophilized. Contains PBS.
Other Product Data	UniProt link P43488: OX40L (mouse) FLAG is a registered trademark of Sigma-Aldrich Co.
Shipping and Handling
Shipping	BLUE ICE
Short Term Storage	+4°C
Long Term Storage	-20°C
Handling Advice	After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution. PBS containing at least 0.1% BSA should be used for further dilutions.
Use/Stability	Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.
Documents
MSDS	Download PDF
Product Specification Sheet
Datasheet	Download PDF

Product-specific References

Description

OX40L [CD252; CD134L] is a membrane-expressed cytokine that belongs to the TNF ligand family. It acts as costimulator through interaction with its receptor OX40 on T cells, stimulating T cell activation, proliferation and cytokine production. OX40L is expressed on antigen presenting cells including B cells, dendritic cells, mast cells and endothelium. MultimericOX40L is a high activity construct in which two trimeric OX40L ligands are artificially linked via the collagen domain of ACRP30. This construct very effectively activates T cell proliferation.

Product References

Endothelial cell co-stimulation through OX40 augments and prolongs T cell cytokine synthesis by stabilization of cytokine mRNA: J. Mestas, et al.; Int. Immun. 17, 737 (2005)
Tumor necrosis factor receptor family costimulation increases regulatory T-cell activation and function via NF-κB: M. Lubrano di Ricco, et al.; Eur. J. Immunol. 50, 972 (2020)

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