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Chimerigen
IL-2 (mouse):Fc (mouse) (rec.) (non-lytic)
250
CHF
CHF 250.00
In stock
CHI-MF-12002-C01010 µgCHF 250.00
CHI-MF-12002-C05050 µgCHF 430.00
| Product Details | |
|---|---|
| Synonyms | Interleukin-2; Aldesleukin |
| Product Type | Protein |
| Properties | |
| Source/Host | NS1 cells |
| Sequence |
The extracellular domain of mouse IL-2 (aa 21-169) is fused to the N-terminus of the Fc region of a mutant mouse IgG2a. |
| Crossreactivity | Mouse |
| Biological Activity |
Specific Activity: 1-1.5 x106 Units/mg, measured using CTLL indicator cells. |
| MW | ~46kDa (monomer) |
| Purity | ≥98% (SDS-PAGE) |
| Endotoxin Content | <0.06EU/μg protein (LAL test; Lonza). |
| Reconstitution | Reconstitute at 100μg/ml in sterile PBS. |
| Formulation | Lyophilized from 0.2μm-filtered solution in PBS. |
| Protein Negative Control | |
| Other Product Data |
Non-lytic: Acts as a long lasting fusion protein which only binds to the receptor. Mutations to the complement (C1q) and FcgR I binding sites of the IgGs Fc fragment render the fusion proteins incapable of antibody directed cytotoxicity (ADCC) and complement directed cytotoxicity (CDC). |
| Declaration | Manufactured by Chimerigen. |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice |
Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution. |
| Use/Stability |
Stable for at least 1 year after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C. |
| Documents | |
| MSDS | Inquire |
| Product Specification Sheet | |
| Datasheet |
 Download PDF |
Description
Interleukin-2 (IL-2) is a 133 amino acid glycoprotein with one intramolecular disulfide bond and variable glycosylation. It is secreted by activated T cells and induces proliferation and maturation of activated T cells, natural killer cells, and lymphokine activated killer cells. IL-2 also stimulates proliferation of antibody-producing B cells, activates neutrophils, and induces mononuclear cells to secrete IFN-γ and TNF-α and -β. Moreover, studies have shown that IL-2 is required for activation-induced apoptosis, an important hemeostatic mechanism in the immune system, which is involved in the maintenance of peripheral tolerance to self-antigens.
Product References
- Administration of noncytolytic IL-10/Fc in murine models of lipopolysaccharide-induced septic shock and allogeneic islet transplantation: X.X. Zheng, et al.; J. Immunol. 154, 5590 (1995)
- Augmentation and suppression of immune responses to an HIV-1 DNA vaccine by plasmid cytokine/Ig administration: D.H. Barouch, et al.; J. Immunol. 161, 1875 (1998)
- IL-2 receptor-targeted cytolytic IL-2/Fc fusion protein treatment blocks diabetogenic autoimmunity in nonobese diabetic mice: X.X. Zheng, et al.; J. Immunol. 163, 4041 (1999)
- Effects of Cyclosporine on Transplant Tolerance: The Role of IL-2: X.X. Zheng, et al.; Am. J. Transplant. 7, 1907 (2007)
- Single-cell quantification of IL-2 response by effector and regulatory T cells reveals critical plasticity in immune response: O. Feinerman, et al.; Mol. Sys. Biol. 6, 437 (2010)
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