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AdipoGen Life Sciences
IL-21 (mouse) (monomeric):Fc (LALA-PG)-KIH (human) (rec.)
440
CHF
CHF 440.00
In stock
AG-40B-0250-C05050 µgCHF 440.00
NEW
Figure 1: Protein structure of IL-21 (mouse) (monomeric):Fc (LALA-PG)-KIH (human) (rec.) (Prod. No. AG-40B-0250).
Figure 2: IL-21 (mouse) (monomeric):Fc (LALA-PG)-KIH (human) (rec.) (#AG-40B-0250) is more active than a IL-21 (mouse):Fc (mouse) (rec.) (non-lytic) from a competitor. Method: 5x10^4 CD4+ T cells are treated with IL-21 (mouse) (monomeric):Fc (LALA-PG)-KIH (human) (rec.) (#AG-40B-0250), IL-21 (mouse):Fc (mouse) (rec.) (non-lytic) from a competitor, or Fc (LALA-PG)-KIH (human) IgG1 Control (rec.) (#AG-35B-0018) at different concentrations indicated on the figure for 30 minutes. Cells are lysed and cell extracts are separated by SDS-PAGE (12.5%) under reducing conditions, transferred to nitrocellulose overnight and incubated with anti-Phospho-STAT3 (Tyr705) (human), Rabbit Monoclonal (RM261) (REV-31-1142-00) (1/1000). After the addition of an anti-rabbit secondary antibody coupled to HRP (1/10’000), proteins are visualized by a chemiluminescence detection system. The figure shows the quantification of the western blot experiment in relative units (RU).
| Product Details | |
|---|---|
| Synonyms | Interleukin-21 (mouse) (monomeric):Fc (LALA-PG) Knobs-into-Holes (human) (rec.) |
| Product Type | Protein |
| Properties | |
| Source/Host | CHO cells. Produced using animal component-free medium. |
| Sequence |
Mouse IL-21 (aa 18-146) is fused at the C-terminus to the Fc portion of human IgG1 (LALA-PG) (Knobs-into-Holes technology) (see reference: J.B. Ridgway, et al.; Protein Eng. 9, 617 (1996)). |
| Crossreactivity |
Human Mouse |
| Biological Activity |
Activates STAT3 phosphorylation in human and mouse CD4+ T cells at an ED50 <0.5 ng/ml. |
| MW | ~54kDa and 28kDa (SDS-PAGE) |
| Purity | ≥95% (SDS-PAGE) |
| Endotoxin Content | <0.02EU/μg protein (LAL test). |
| Concentration | After reconstitution: 1mg/ml |
| Reconstitution | Reconstitute with 50μl endotoxin-free water. |
| Accession Number | Q9ES17 |
| Formulation | Lyophilized. Contains PBS. |
| Protein Negative Control |
Fc (LALA-PG)-KIH (human) IgG1 Control (rec.) (Prod. No. AG-35B-0018) |
| Other Product Data |
Uniprot link Q9ES17: IL-21 (mouse) |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice |
After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution. PBS containing at least 0.1% BSA should be used for further dilutions. |
| Use/Stability |
Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C. |
| Documents | |
| MSDS | Inquire |
| Product Specification Sheet | |
| Datasheet |
 Download PDF |
Description
IL-21 is a monomeric 15 kDa cytokine, identified as a multifunctional cytokine principally produced by follicular helper T (Tfh), T helper 17 (Th17) and natural killer (NK) cells, but also by CD8+ T cells. As a receptor for IL-21, IL-21R, a class I cytokine heterodimeric receptor, shares a common cytokine receptor γ chain with other cytokine families, including IL-2, IL-4, IL-7, IL-9 and IL-15. The IL-21 receptor (IL-21R) is heterodimeric (composed of an α and ɣ-chain) and physiologically expressed on immune cells (T and B cells, macrophages, DCs, thymocytes and splenocytes). Upon binding to its receptor, IL-21 induces strong and sustained activation of STAT3, which is critical for its effects on B cell and T cell differentiation. IL-21 produced by follicular helper CD4 T cells (TFH) acts directly on B cells to generate high-affinity, class-switched antibodies. IL-21 is important for the maturation of T and B cells in Germinal Centers (GCs) and supports the formation of antigen-specific memory B cells and long-lived plasma cells. The formation of Th17 cells is promoted by IL-21. IL-21 is an important key factor in chronic inflammatory and in autoantibody-mediated skin diseases, where Th17, Th1, Tfh or B cells are critically involved.
IL-21 is a promising immunotherapeutic agent for cancer. CD8 T cells expressing IL-21R fight cancer and drive autoimmune disease. IL-21 promotes maturation, enhances cytotoxicity, and induces the production of IFN- γ and perforin by NK cells. IL-21 is known to directly induce apoptosis in certain types of lymphoma. Finally, IL-21 is used in adoptive transfer of in vitro expanded tumor antigen-specific CD8 + T cells (TILs cells) and IL-21 induced a long-lived memory phenotype compared to the cells not treated with IL-21.
The protein IL-21 (mouse) (monomeric):Fc (LALA-PG)-KIH (human) (rec.) is produced by using two different vectors, one encoding for the IL-21 (mouse):Fc (LALA-PG) Knobs sequence (synthesizing a protein of 60kDa) and one encoding for the Fc (LALA-PG) Holes sequence (synthesizing a protein of 28kDa). Both vectors transfected into CHO cells produce both Fc (LALA-PG) molecules (Knobs-into-Holes technology; J.B. Ridgway, et al.; Protein Eng. 9, 617 (1996)) required for dimerization of the Fc moieties and for secretion of the final protein IL-21 (mouse) (monomeric):Fc (LALA-PG)-KIH (human). The LALA-PG mutations inhibit binding to FcγRs and C1q while FcRn binding and Fc stability remain unaffected.
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