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AdipoGen Life Sciences
Fc (LALA-PG)-KIH (human) IgG1 Control (rec.)
340
CHF
CHF 340.00
In stock
AG-35B-0018-C05050 µgCHF 340.00
Figure 1: Protein structure of Fc (LALA-PG)-KIH (human) IgG1 Control (rec.) (Prod. No. AG-35B-0018).
Figure 2: Fc (LALA-PG)-KIH (human) IgG1 Control (rec.) (#AG-35B-0018) does not bind to the human Fcg Receptor I. Method: Fcg Receptor I (mouse) is coated on an ELISA plate at 1 µg/ml overnight at RT. Fc (LALA-PG)-KIH (human) IgG1 Control (rec.), a control Fc-KIH (human) IgG1 Control (rec.) (#AG-35B-0015), or a classical Fc (human) IgG1 Control (rec.) (#AG-35B-0007) are added (starting at 4000 ng/ml with a two-fold serial dilution) during one hour and then detected using an anti-human Fc (HRP) antibody. Both controls proteins bind to the mouse Fcg Receptor, while the Fc (LALA-PG)-KIH (human) IgG1 Control (rec.) shows no binding as expected with its LALA-PG mutations.
| Product Details | |
|---|---|
| Synonyms | Fc (LALA-PG) Knobs-into-Holes (human) IgG1; Immunoglobulin 1 Fragment Constant |
| Product Type | Protein |
| Properties | |
| Source/Host | HEK 293 cells. Produced using animal component-free medium. |
| Sequence |
Fc (LALA-PG) (human) IgG1 (Knobs-into-Holes Technology) (see reference: J.B. Ridgway, et al.; Protein Eng. 9, 617 (1996)) |
| Crossreactivity |
Human Mouse |
| Specificity |
Fc (LALA-PG)-KIH (human) does not bind to FcRs. |
| MW | ~28kDa (SDS-PAGE) |
| Purity | ≥95% (SDS-PAGE) |
| Endotoxin Content | <0.01EU/μg purified protein (LAL test). |
| Concentration | After reconstitution: 1mg/ml |
| Reconstitution | Reconstitute with 50µl endotoxin-free water. |
| Accession Number | P01857 |
| Formulation | Lyophilized. Contains PBS. |
| Other Product Data |
Uniprot: P01857 |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice |
After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution. PBS containing at least 0.1% BSA should be used for further dilutions. |
| Use/Stability |
Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C. |
| Documents | |
| MSDS | Inquire |
| Product Specification Sheet | |
| Datasheet |
 Download PDF |
Description
Negative control in experiments with recombinant proteins fused to human IgG1 Fc (LALA-PG)-KIH (Knobs-into-Holes) (e.g. Prod. No. AG-40B-0248). This protein is not an appropriate negative control for other standard Fc protein constructs.
The protein Fc (LALA-PG)-KIH (human) IgG1 Control (rec.) is produced by using two different vectors, one encoding for the Fc (LALA-PG) Knobs sequence with a small linker (synthesizing a protein of 29kDa) and one encoding for the Fc (LALA-PG) Holes sequence (synthesizing a protein of 28kDa). Both vectors transfected into HEK293 cells produce both Fc molecules (Knobs-into-Holes technology; J.B. Ridgway, et al.; Protein Eng. 9, 617 (1996)) required for dimerization and for secretion of the final protein Fc (LALA-PG)-KIH (human) IgG1 Control (rec.). The LALA-PG mutations inhibit binding to FcγRs and C1q while FcRn binding and Fc stability remain unaffected.
InVivoKines™ are a new generation of recombinant fusion proteins for immunotherapeutic, preclinical and translational in vivo research, developed and manufactured by AdipoGen Life Sciences.
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