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ODN 2243 (Control for ODN 2216) Endotoxin-free (sterile)

IAX-200-205-C100 100 µg INQ
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Product Data
Synonyms Negative Control for ODN 2216 (Cat. No.: IAX-200-005)
Properties
MW 6,684g/mol
Sequence 5'-ggGGGAGCATGCTGgggggg-3' (lower case letters indicate phosphorothioate linkage).
Reconstitution For a 100μM stock solution, dissolve total vial content in 150μl (100μg size) sterile endotoxin-free water or PBS.
Formulation Lyophilized. Sterile.
Biological Activity Negative control GpC ODN for in vivo use in rodents (50-150μg per injection).
As active compound use CpG ODN 2216 (Cat. No.: IAX-200-005).
For negative control without agonistic nor antagonistic activity: Neutral-ODN (Cat. No.: IAX-200-202).
Crossreactivity Human, Mouse, Rat
Specificity Human, Mouse, Rat
Endotoxin Content <0.002EU/μg
Origin Manufactured by Innaxon.
Other Product Data Contains: 100μg size includes 1.5ml ddWater Endotoxin-free (sterile) (Cat. No.: IAX-900-002-LD15).
Reconstitution Note: Add 50% of solvent and let dissolve for 10min. Add remaining 50% of the solvent and mix thoroughly. Moderate warming may aid dissolving.

Click here for Original Manufacturer Product Datasheet: Our product description may differ slightly from the original manufacturers product datasheet.
Product Type Chemical
Shipping and Handling
Shipping AMBIENT
Short Term Storage +4°C
Long Term Storage +4°C
Handling Advice Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and keep aqueous stock solutions for 1 day at 4°C or store at -20°C (shelf-life 6 months).
Use/Stability Stable for at least 2 years after receipt when stored at +4°C.
Documents
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Product Description

Unmethylated CG dinucleotides within particular sequence contexts are responsible for the immunostimulatory activity of bacterial DNA. Synthetic oligonucleotides (ODN) that contain such CpG motifs (CpG ODNs) mimic microbial DNA. The innate immune system of vertebrates has the ability to recognize CpG motifs in microbial DNA via the Toll-like receptor (TLR) 9 if the CpG ODN were free of additional immune stimulatory contaminants often present in synthetic commercial CpG ODN preparations designed for molecular biology applications (i.e. PCR). Given that high quality CpG ODNs were used [i.e. endotoxin-free], a close link has been established between the expression of TLR9 on certain immune cell subsets and the modulation of the immune system by CpG DNA. Different types of CpG ODNs were identified based on their differing biological effects on different cell types: ODN Type A is a potent inducer of IFN-α in human PDC, (i.e. ODN 1585 or 2216) leading to antigen presenting cell (APC) maturation, whereas ODN Type B (i.e. ODN 2006 or ODN 1668 / ODN 1826) is a weak inducer of IFN-α but rather stimulates IL-8 production and increasing costimulatory and Ag-presenting molecules and triggers proliferation of B-cells and IgM and IL-6 production. A third type of CpG ODN has been identified, termed ODN Type C, with both high induction of INF-α in PDC and activation of B-cells. The sequence of CpG Type C (also called K) (i.e. ODN 2395 or M362) combines elements of both Type A and Type B and contains a central palindromic sequence with CG dinucleotides, a characteristic feature of Type A, and a TCGTCG motif at the 5' end, present in Type B CpG ODNs. Although the CpG motifs are thought to differ between mice and humans, in both species the recognition of CpG ODNs is mediated by TLR9. The optimal CpG motif in humans is GTCGTT and GACGTT for the murine sequence. However, recent evidence suggests that this sequence specificity is restricted to phosphorothioate (PS)-modified ODN and is not observed when a natural phosphodiester backbone is used. In recent years sequence requirements, specificity, signalling pathways and kinetics of the TLR9 suppression by inhibitory ODNs (iODNs) have been investigated.

Product References

  1. Comparison of different CpG oligodeoxynucleotide classes for their capability to stimulate human NK cells: S. Sivori, et al.; Eur. J. Immunol. 36, 961 (2006) 
  2. Innate immune responses induced by classes of CpG oligodeoxynucleotides in ovine lymph node and blood mononuclear cells: J.S. Booth, et al.; Vet. Immunol. Immunopathol. 115, 24 (2007) 
  3. Impact of class A, B and C CpG-oligodeoxynucleotides on in vitro activation of innate immune cells in human immunodeficiency virus-1 infected individuals: J.A. Martinson, et al.; Immunology 120, 526 (2007) 
  4. Three CpG oligodeoxynucleotide classes differentially enhance antigen-specific humoral and cellular immune responses in mice: Y. Liu, et al.; Vaccine 29, 5778 (2011) 
  5. Toll-like receptor and IL-12 signaling control susceptibility to contact hypersensitivity: S.F. Martin, et al.; J. Exp. Med. 205, 2151 (2008)
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