|Synonyms||Inhibitory ODN (iODN): Class II|
|Sequence||5’-tttagggttagggttagggttaggg-3’ (lower case letters: phosphorothioate linkage: nuclease resistant)|
|Reconstitution||For a 100μM stock solution, dissolve total vial content in 1210μl (1mg size) or 121μl (100μg size) sterile endotoxin-free water or PBS.|
|Biological Activity||Potent sequence of an inhibitory ODN for in vivo use in rodents (100-300µg per injection), prototype class II, inhibits STAT signaling, independent of TLR signaling.
Negative control: Neutral-ODN (Cat. No.: IAX-200-202).
|Origin||Manufactured by Innaxon.|
|Other Product Data||Contains: 100μg size includes 1.5ml ddWater Endotoxin-free (sterile) (Cat. No.: IAX-900-002-LD15). 1mg and 3 x 1mg sizes each include 10ml ddWater Endotoxin-free (sterile) (Cat. No.: IAX-900-002-L010).
Reconstitution Note: Add 50% of solvent and let dissolve for 10min. Add remaining 50% of the solvent and mix thoroughly. Moderate warming may aid dissolving.
Click here for Original Manufacturer Product Datasheet: Our product description may differ slightly from the original manufacturers product datasheet.
|Shipping and Handling|
|Short Term Storage||+4°C|
|Long Term Storage||+4°C|
|Handling Advice||Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and keep aqueous stock solutions for 1 day at 4°C or store at -20°C (shelf-life 6 months).|
|Use/Stability||Stable for at least 2 years after receipt when stored at +4°C.|
Class I: G-stretch ODNs: TLR9-specific competitors, some iODNs may also affect TLR7 and TLR8 signaling
Class II: ODNs with telomeric repeats: TLR-independent inhibitors of STAT signaling (cellular uptake via an 'ODN receptor'?)
Class III: Inhibitors of DNA uptake in a sequence independent manner
Class IV: Long phosphorothioate ODNs as direct competitors of TLR9 signaling in a sequence independent manner