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LPS from S. minnesota R3 (Rd2) TLRpure Sterile Solution

IAX-100-021-C500 500 µg INQ
IAX-100-021-M001 1 mg INQ
IAX-100-021-5001 5 x 1 mg INQ
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Additional Information

Product Data
Synonyms Lipopolysaccharide (LPS) from S. minnesota strain R3 (Rd2), R-type (rough/mutant) LPS
Properties
Source/Host Isolated and purified from S. minnesota strain R3.
Purity ≥99.9%. No detectable DNA, RNA and protein traces.
Formulation Liquid. Colourless clear aqueous solution.
Concentration 1mg/ml stabilised in sterile, double-distilled water (ddWater), without any additives.
Biological Activity Optimal concentration is dependent upon cell type, species, desired activation and analysis: 0.05-1.0μg/ml. Does not activate any TLR other than TLR4 as tested up to 50μg/ml in relevant cellular systems (macrophages).
Origin Manufactured by Innaxon.
Other Product Data TLRpure™: • Qualified Purity & Activity • High potency TLR4-specific Ligands • Ultrapure (no detectable protein, RNA and DNA) • Tested on TLR4 KO murine macrophages • Standardised Aqueous Sterile Solutions • No purification or hazardous solubilisation • Excellent lot-to-lot consistency

Click here for Original Manufacturer Product Datasheet: Our product description may differ slightly from the original manufacturers product datasheet.
Product Type Chemical
Shipping and Handling
Shipping AMBIENT
Short Term Storage +4°C
Long Term Storage +4°C
Handling Advice Do not freeze. To yield a 100μg/ml (1,000-100x) stock solution add 100μl of LPS to 900μl endotoxin-free and sterile ddWater (Cat. No.: IAX-900-002) or 0.9% NaCl Solution (Cat. No.: IAX-900-003) and mix well. Prepare diluted LPS working solutions just prior to use, keep sterile. Ready-made solution is cell culture-grade.
Use/Stability Stable for at least 2 years after receipt when stored at +4°C.
Documents
MSDS Download Document Download

Product Description

Activation of cells by LPS is mediated by the Toll-like receptor 4 (TLR4). For optimal interaction with LPS, TLR4 requires association with myeloid differentiation protein 2 (MD-2). According to current consensus activation of TLR4 is preceded by the transfer of LPS to membrane-bound (m) or soluble (s) CD14 by LPS-binding protein (LBP). Re-form LPS and lipid A, but not S-form LPS, are capable of inducing TNF-α responses also in the absence of CD14. LPS, synthesized by most wild-type (WT) Gram-negative bacteria (S-form LPS), consists of three regions, the O-polysaccharide chain, which is made up of repeating oligosaccharide units, the core oligosaccharide and the lipid A, which harbors the endotoxic activity of the entire molecule. R-form LPS synthesized by the so-called rough (R) mutants of Gram-negative bacteria lacks the O-specific chain. Furthermore, the core-oligosaccharide may be present in different degrees of completion, depending on the class (Ra to Re) to which the mutant belongs. LPS are amphipathic molecules whose hydrophobicity decreases with increasing length of the sugar part. Based upon these differences, S- and R-form LPS show marked differences in the kinetics of their blood clearance and cellular uptake as well as in the ability to induce oxidative burst in human granulocytes and to activate the host complement system.

Product References

  1. A new method for the extraction of R lipopolysaccharides: C. Galanos, et al.; Eur. J. Biochem. 9, 245 (1969)
  2. Electrodialysis of lipopolysaccharides and their conversion to uniform salt forms: C. Galanos & O. Lüderitz; Eur. J. Biochem. 54, 603 (1975)
  3. Immunoblot analysis of the R-form lipopolysaccharide from Salmonella S forms: S. Schlecht, et al.; Zentralbl. Bakteriol. 277, 288 (1992)
  4. Isolation and purification of R-form lipopolysaccharides: C. Galanos & O. Lüderitz; Methods in Carbohydrate Chem. 9, 11 (1993)
  5. The influence of low growth temperature on the amount of free R lipopolysaccharide, on the expression of R-core determinants and on O-chainlengths in Salmonella S forms: S. Schlecht & H. Mayer; Zentralbl. Bakteriol. 280, 448 (1994)
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