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Chimerigen
IL-7 (human) (rec.) (His)
250
CHF
CHF 250.00
In stock
CHI-HF-20107-C01010 µgCHF 250.00
CHI-HF-20107-C05050 µgCHF 720.00
| Product Details | |
|---|---|
| Synonyms | Interleukin-7; Lymphopoietin 1; LP-1 |
| Product Type | Protein |
| Properties | |
| Source/Host | HEK 293 cells |
| Sequence | The extracellular domain of human IL-7 (aa 26-177) is fused at the C-terminus to a His-tag. |
| Crossreactivity | Human |
| Biological Activity | Measured by the dose dependent stimulation of the proliferation of murine 2E8 cells. The ED50 for this effect is typically 0.2ng/ml corresponding to a specific activity of 5x 106 units/mg. |
| MW | ~27kDa (SDS-PAGE) |
| Purity | ≥95% (SDS-PAGE) |
| Endotoxin Content | <0.01EU/μg protein (LAL test; Lonza). |
| Reconstitution |
Reconstitute 10µg vial with 100 µl sterile water to a concentration of 0.1mg/ml. Reconstitute 50µg vial with 100 µl sterile water to a concentration of 0.5mg/ml. Add 1X PBS to the desired protein concentration. |
| Formulation | Lyophilized from 0.2μm-filtered solution in PBS. |
| Other Product Data | NCBI reference AAH47698.1: IL-7 (human) |
| Declaration | Manufactured by Chimerigen. |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice |
Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution. PBS containing at least 0.1% BSA should be used for further dilutions. |
| Use/Stability |
Stable for at least 1 year after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C. |
| Documents | |
| MSDS | Inquire |
| Product Specification Sheet | |
| Datasheet |
 Download PDF |
Description
Interleukin-7 (IL-7), is a hematopoietic growth factor and a member of the IL-7/IL-9 family. IL-7 stimulates the proliferation of lymphoid progenitors and is secreted by stromal cells in the bone marrow and thymus. IL-7 is also important for proliferation during certain stages of B cell maturation. IL-7 and the hepatocyte growth factor (HGF) form a heterodimer that functions as a pre-pro-B cell growth-stimulating factor. It is found to be a cofactor for V(D)J rearrangement of the T cell receptor β (TCRβ) during early T cell development. IL-7 can be produced locally by intestinal epithelial and epithelial goblet cells and may serve as a regulatory factor for intestinal mucosal lymphocytes.
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