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Chemodex
Nitrotetrazolium blue chloride
26
CHF
CHF 26.00
In stock
CDX-N0009-M100100 mgCHF 26.00
CDX-N0009-G0011 gCHF 122.00
| Product Details | |
|---|---|
| Synonyms | NBT; p-Nitro-Blue tetrazolium chloride; p-Nitrotetrazolium blue; Nitro BT |
| Product Type | Chemical |
| Properties | |
| Formula |
C40H30Cl2N10O6 |
| MW | 817.2 |
| CAS | 298-83-9 |
| RTECS | XF8045000 |
| Source/Host Chemicals | Synthetic. |
| Purity Chemicals | ≥95% (NMR) |
| Appearance | Yellow powder. |
| Solubility | Soluble in water. |
| Identity | Determined by NMR. |
| Declaration | Manufactured by Chemodex. |
| Other Product Data |
Click here for Original Manufacturer Product Datasheet |
| InChi Key | FSVCQIDHPKZJSO-UHFFFAOYSA-L |
| Smiles | [Cl-].[Cl-].COC1=CC(=CC=C1[N+]1=NC(=NN1C1=CC=C(C=C1)[N+]([O-])=O)C1=CC=CC=C1)C1=CC=C(C(OC)=C1)[N+]1=NC(=NN1C1=CC=C(C=C1)[N+]([O-])=O)C1=CC=CC=C1 |
| Shipping and Handling | |
| Shipping | AMBIENT |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice |
Keep cool and dry. Protect from light and moisture. |
| Use/Stability | Stable for at least 2 years after receipt when stored at -20°C. |
| Documents | |
| MSDS | Inquire |
| Product Specification Sheet | |
| Datasheet |
 Download PDF |
Description
NADPH-diaphorase substrate that competitively inhibits NOS (nitric oxide synthase). Well-known scavenger of superoxide anions. Dye that is used for detection of alkaline phosphatase in combination with 5-bromo-4-chloro-3-indoxyl phosphate (BCIP). This substrate system produces an insoluble NBT diformazan end product that is blue in color and can be observed visually. When used with BCIP, it is suitable for detection of alkaline phosphatase in western blots, for immunohistological staining procedures and for colorimetric indication of bacterial infection in blood samples. Used as a redox indicator for enzymatic reactions including dehydrogenases, threonine deaminase, glucose-6-phosphate dehydrogenase (G6PDH), phosphofructokinase on polyacrylamide gels, oxidases on polyacrylamide gels and pentose shunt dehydrogenses. The NBT/BCIP reaction is also used for colorimetric/spectrophotometric activity assays of oxidoreductases. One application is in activity stains in gel electrophoresis, such as with the mitochondrial electron transport chain complexes.
Product References
(1) D.G. Nathan, et al.; J. Clin. Invest. 48, 1895 (1969) | (2) J. McGadey; Histochem. Histochem. Histochim. 23, 180 (1970) | (3) R. Freeman & B. King; J. Clin. Pathol. 25, 912 (1972) | (4) D.A. Knecht & R.L. Dimond; Anal. Biochem. 136, 180 (1984) | (5) Y. Nisimoto, et al.; J. Biol. Chem. 261, 285 (1986) | (6) K. Nichols, et al.; Neuroscience 51, 791 (1992) | (7) C. Libon, et al.; J. Leukoc. Biol. 53, 93 (1993) | (8) C.K. Mittal; Biochem. Biophys. Res. Commun. 193, 126 (1993) | (9) M. De la Fuente, et al.; Comp. Immunol. Microbiol. Infect. Dis. 16, 29 (1993) | (10) A. Pistelli, et al.; Biochem. Pharmacol. 47, 1737 (1994) | (11) A. Trinhle, et al.; BioTechniques 42|756 (2007) | (12) J.M. Ross; J. Visual. Exp. 57, e3266 (2011) | (13) Z.A. Siddiqi, et al.; Eur. J. Med. Chem. 57, 102 (2012)
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