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Chemodex
N-(5-Fluoresceinyl)-maleinimide
Specifications / Handling
| Product Details | |
|---|---|
| Synonyms | N-(5-Fluoresceinyl)-maleimide; Fluorescein-5-maleimide; 1-(3',6'-Dihydroxy-3-oxo-3H-spiro[2-benzofuran-1,9'-xanthen]-5-yl)-1H-pyrrole-2,5-dione |
| Product Type | Chemical |
| Properties | |
| Formula | C24H13NO7 |
| MW | 427.36 |
| CAS | 75350-46-8 |
| Source/Host Chemicals | Synthetic. |
| Purity Chemicals | ≥90% (HPLC) |
| Appearance | Orange to brown solid. |
| Solubility | Soluble in DMF. |
| Identity | Determined by 1H-NMR. |
| Declaration | Manufactured by Chemodex. |
| Other Product Data |
Click here for Original Manufacturer Product Datasheet |
| InChi Key | AYDAHOIUHVUJHQ-UHFFFAOYSA-N |
| Smiles | OC1=CC2=C(C=C1)C1(OC(=O)C3=C1C=CC(=C3)N1C(=O)C=CC1=O)C1=C(O2)C=C(O)C=C1 |
| Shipping and Handling | |
| Shipping | AMBIENT |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice | Protect from light and moisture. |
| Use/Stability | Stable for at least 2 years after receipt when stored at -20°C. |
| Documents | |
| Safety Data Sheet |
Download PDF |
| Product Specification Sheet | |
| Datasheet |
Download PDF |
Scientific Background Information
Green fluorescent thiol-reactive dye (Ex/Em: 494/519nm) for bioconjugation, widely used as protein and peptide fluorescent labeling reagent, easily conjugating fluorescein. The reaction requires very mild condition and is highly specific. The maleimide group reacts optimally with sulfhydryl groups on cysteine side chains at pH 7, forming a stable thioether bond. The compound can be used to label proteins for the detection of conformational changes, assembly of multisubunit complexes, and ligand-binding processes.
(1) Anal. Lett. 18, 393 (1985) | (2) Biochem.; 30, 2113 (1991)
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