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Chemodex
5-DTAF hydrochloride
Specifications / Handling
| Product Details | |
|---|---|
| Synonyms | 5-[(4,6-Dichlorotriazin-2-yl)amino]fluorescein hydrochloride; NSC378139 |
| Product Type | Chemical |
| Properties | |
| Formula | C23H12Cl2N4O5 . HCl |
| MW | 495.3 . 36.5 |
| CAS | 21811-74-5 |
| Source/Host Chemicals | Synthetic. |
| Purity Chemicals | ≥98% (HPLC) |
| Appearance | Yellow to orange powder. |
| Solubility | Soluble in chloroform. |
| Identity | Determined by NMR. |
| Declaration | Manufactured by Chemodex. |
| Other Product Data |
Click here for Original Manufacturer Product Datasheet |
| InChi Key | CEKSHCAIHGNWBK-UHFFFAOYSA-N |
| Smiles | Cl.OC(=O)C1=C(C=CC(NC2=NC(Cl)=NC(Cl)=N2)=C1)C1=C2C=CC(=O)C=C2OC2=CC(O)=CC=C12 |
| Shipping and Handling | |
| Shipping | AMBIENT |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice |
Keep cool and dry. Protect from light and moisture. |
| Use/Stability | Stable for at least 2 years after receipt when stored at -20°C. |
| Documents | |
| Safety Data Sheet |
Download PDF |
| Product Specification Sheet | |
| Datasheet |
Download PDF |
Scientific Background Information
5-DTAF is highly reactive with proteins. Unlike other reactive fluoresceins, 5-DTAF not only reacts with amino groups, but also reacts with thiol groups and even directly reacts with hydroxy groups such as polysaccharides and other alcohols in aqueous solution at pH above 9. Due to its high reactivity, 5-DTAF is also used to label carbohydrates. Spectral properties: Abs/Em = 492/517 nm
(1) D. Blakeslee & M.G. Baines; J. Immunol. Methods 13, 305 (1976) | (2) D. Blakeslee; J. Immunol. Methods 17, 361 (1977) | (3) R. Siegler, et al.; J .Pharm. Biomed. Anal. 7, 45 (1989) | (4) S. Prigent-Richard, et al.; J. Biomed. Mater. Res. 40, 275 (1998) | (5) K.N. Krahn, et al.; Anal. Biochem. 350, 177 (2006) | (6) M. Ahmed, et al.; J. Ind. Microbiol. Biotechnol. 38, 249 (2011) | (7) S.E. Szczesny, et al.; PLoS One 9, e99588 (2014)
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