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5-FAM

CDX-C0014-M025 25 mg INQ
CDX-C0014-M100 100 mg INQ
CDX-C0014-G001 1 g INQ
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Additional Information

Product Data
Synonyms 5-Carboxyfluorescein
Properties
Formula C21H12O7
MW 376.32
CAS 76823-03-5
Source/Host Synthetic.
Purity ≥99% (HPLC)
Appearance Yellow-orange solid.
Solubility Soluble in water, DMSO or DMF.
Identity Determined by 1H-NMR.
Origin Manufactured by Chemodex.
Other Product Data Click here for Original Manufacturer Product Datasheet
Our product description may differ slightly from the original manufacturers product datasheet.
InChi Key VPMWLNJETPKSSC-UHFFFAOYSA-N
Product Type Chemical
Shipping and Handling
Shipping AMBIENT
Short Term Storage +4°C
Long Term Storage +4°C
Handling Advice Protect from light and moisture.
Use/Stability Stable for at least 2 years after receipt when stored at +4°C.

Product Description

The amine-reactive fluorescein derivatives are probably the most common fluorescent derivatization reagents for covalently labeling proteins. Carboxyfluorescein is better retained in cells than is fluorescein, its pKa of ~6.5 is lower than the cytosolic pH of most cells (pH ~6.8-7.4). Carboxyfluorescein is commonly employed as a polar tracer.

Product References

(1) R. Fischer et al., Bioconjugate Chem. 14, 653 (2003) | (2) E. Ralston et al., Biochim. Biophys. Acta, Biomembranes 649(1), 133 (1981)
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