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Hoechst 33258

CDX-B0029-M500 500 mg INQ
CDX-B0029-G005 5 g INQ
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Additional Information

Product Data
Synonyms 4-[5-(4-Methyl-1-piperazinyl)-1H,1'H-2,5'-bibenzimidazol-2'-yl]phenol trihydrochloride
Formula C25H24N6O . 3HCl
MW 533.88
CAS 23491-45-4
Source/Host Synthetic.
Purity ≥98% (HPLC, TLC)
Appearance Yellow solid.
Solubility Soluble in water.
Identity Determined by 1H-NMR.
Origin Manufactured by Chemodex.
Other Product Data Click here for Original Manufacturer Product Datasheet
Our product description may differ slightly from the original manufacturers product datasheet.
Product Type Chemical
Shipping and Handling
Shipping AMBIENT
Short Term Storage +4°C
Long Term Storage +4°C
Handling Advice Protect from light and moisture.
Use/Stability Stable for at least 2 years after receipt when stored at +4°C.

Product Description

Useful for staining DNA, chromosomes and nuclei. Binds to the minor groove of DNA at AT-rich sequences. This dye is commonly used for determining the DNA content of viable cells without detergent treatment or fixation and for fluorescence microscopy or flow cytometry.

Product References

(1) A. Kumar et al.; In Vitro Cell. & Developm. Biol. 44(7), 189 (2008) | (2) D. Weidmann et al.; Meth. Enzym. 446, 277 (2008) | (3) D. Plesca et al.; Meth. Enzym. 446, 107 (2008) | (4) K.H. Elstein et al.; Exp. Cell. Res. 211, 322 (1994) | (5) Y.-J. Kim et al.; Anal. Biochem. 174, 168 (1988) | (6) C. Labarca et al.; Anal. Biochem. 102, 344 (1980) | (7) S.A. Latt et al.; J. Histochem. Cytochem. 24, 24 (1976)
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