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Chemodex
DAF-FM
Specifications / Handling
| Product Details | |
|---|---|
| Synonyms | 4-Amino-5-methylamino- 2',7'-difluorofluorescein; Diaminofluorescein-FM |
| Product Type | Chemical |
| Properties | |
| Formula |
C21H14F2N2O5 |
| MW | 412.34 |
| CAS | 254109-20-1 |
| Source/Host Chemicals | Synthetic. |
| Purity Chemicals | ≥98% (HPLC) |
| Appearance | Orange solid. |
| Solubility | Soluble in DMSO. |
| Identity | Determined by 1H-NMR. |
| Declaration | Manufactured by Chemodex. |
| Other Product Data |
Click here for Original Manufacturer Product Datasheet |
| InChi Key | BJTLSPOVXMBXRZ-UHFFFAOYSA-N |
| Smiles | CNC1=C(N)C(C(O)=O)=C(C=C1)C1=C2C=C(F)C(=O)C=C2OC2=C1C=C(F)C(O)=C2 |
| Shipping and Handling | |
| Shipping | AMBIENT |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice | Protect from light and moisture. |
| Use/Stability | Stable for at least 2 years after receipt when stored at -20°C. |
| Documents | |
| Safety Data Sheet |
Download PDF |
| Product Specification Sheet | |
| Datasheet |
Download PDF |
Scientific Background Information
DAF-FM is an important reagent for quantitating low concentrations of nitric oxide in solution. This compound is essentially nonfluorescent until it reacts with NO to form a fluorescent benzotrizole. With excitation/emission maxima of 495/515 nm, DAF-FM can be detected using any instrument that can be used to detect fluorescein.
(1) W.S. Kim et al.; Anal. Chem. 78(6), 1859 (2006) | (2) M.M. Knight et al.; Am. J. Physiol. 284(4, Pt. 1), C1083 (2003) | (3) H. Kojima et al.; Adv. Mat. 12(10), 763 (2002) | (4) H. Kojima et al.; Angew. Chem. 111( 21), 3419 (1999)
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