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DAF-FM

CDX-A0023-M001 1 mg INQ
CDX-A0023-M005 5 mg INQ
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Additional Information

Product Data
Synonyms 4-Amino-5-methylamino- 2',7'-difluorofluorescein; Diaminofluorescein-FM
Properties
Formula C21H14F2N2O5
MW 412.34
CAS 254109-20-1
Source/Host Synthetic.
Purity ≥98% (HPLC)
Appearance Orange solid.
Solubility Soluble in DMSO.
Identity Determined by 1H-NMR.
Origin Manufactured by Chemodex.
Other Product Data Click here for Original Manufacturer Product Datasheet
Our product description may differ slightly from the original manufacturers product datasheet.
InChi Key BJTLSPOVXMBXRZ-UHFFFAOYSA-N
Product Type Chemical
Shipping and Handling
Shipping AMBIENT
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice Protect from light and moisture.
Use/Stability Stable for at least 2 years after receipt when stored at -20°C.

Product Description

DAF-FM is an important reagent for quantitating low concentrations of nitric oxide in solution. This compound is essentially nonfluorescent until it reacts with NO to form a fluorescent benzotrizole. With excitation/emission maxima of 495/515 nm, DAF-FM can be detected using any instrument that can be used to detect fluorescein.

Product References

(1) W.S. Kim et al.; Anal. Chem. 78(6), 1859 (2006) | (2) M.M. Knight et al.; Am. J. Physiol. 284(4, Pt. 1), C1083 (2003) | (3) H. Kojima et al.; Adv. Mat. 12(10), 763 (2002) | (4) H. Kojima et al.; Angew. Chem. 111( 21), 3419 (1999)
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