AdipoGen Life Sciences

DAPT

CHF 90.00
In stock
AG-CR1-0016-M0055 mgCHF 90.00
AG-CR1-0016-M02525 mgCHF 270.00
More Information
Product Details
Synonyms N-[N-(3,5-Difluorophenacetyl-L-alanyl)]-S-phenylglycine tert.butyl ester
Product Type Chemical
Properties
Formula

C23H26F2N2O4

MW 432.5
CAS 208255-80-5
Purity Chemicals ≥98% (NMR)
Appearance White to off-white solid.
Solubility Soluble in 100% ethanol, DMSO (20 mg/ml) or dichloromethane.
Other Product Data

Stock solutions are stable for up to 3 months when stored at -20°C.

InChi Key DWJXYEABWRJFSP-XOBRGWDASA-N
Smiles C[C@H](NC(=O)CC1=CC(F)=CC(F)=C1)C(=O)N[C@H](C(=O)OC(C)(C)C)C1=CC=CC=C1
Shipping and Handling
Shipping AMBIENT
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice After reconstitution, prepare aliquots and store at -20°C.
Protect from light.
Use/Stability Stable for at least 2 years after receipt when stored at -20°C.
Documents
MSDS Download PDF
Product Specification Sheet
Datasheet Download PDF
Description
  • Cell permeable γ-secretase inhibitor (IC50 = 115 nM for total β-amyloid, IC50 = 200 nM for β-amyloid 1-42) [1].
  • Reduces Aβ levels in vivo [2]. 
  • Blocks the proteolytic processing of neurotrophin receptor alike death domain protein (NRADD) [3]. 
  • Does not inhibit persenilinase [4]. 
  • Notch processing inhibitor [5]. 
  • Enhances neuronal differentiation independent of sonic hedgehog (Shh) signaling [6]. 
  • CDK5 activity inhibitor [7]. 
  • Apoptosis enhancer [8]. 
  • Inhibitor of slurp-like1 expression in the floor plate of the neural tube.
Product References
  1. Functional gamma-secretase inhibitors reduce beta-amyloid peptide levels in brain: H.F. Dovey, et al.; J. Neurochem. 76, 173 (2001) 
  2. The gamma-secretase inhibitor N-[N-(3,5-difluorophenacetyl)-L-alanyl]-S-phenylglycine t-butyl ester reduces A beta levels in vivo in plasma and cerebrospinal fluid in young (plaque-free) and aged (plaque-bearing) Tg2576 mice: T.A. Lanz, et al.; J. Pharmacol. Exp. Ther. 305, 864 (2003) 
  3. Release of a membrane-bound death domain by gamma-secretase processing of the p75NTR homolog NRADD: K. Gowrishankar, et al.; J. Cell. Sci. 117, 4099 (2004) 
  4. Presenilin endoproteolysis mediated by an aspartyl protease activity pharmacologically distinct from gamma-secretase: W.A. Campbell, et al.; J. Neurochem. 85, 1563 (2003) 
  5. A gamma-secretase inhibitor blocks Notch signaling in vivo and causes a severe neurogenic phenotype in zebrafish: A. Geling, et al.; EMBO Rep. 3, 688 (2002) 
  6. The notch response inhibitor DAPT enhances neuronal differentiation in embryonic stem cell-derived embryoid bodies independently of sonic hedgehog signaling: T.Q. Crawford & H. Roelink; Dev. Dyn. 236, 886 (2007) 
  7. The Notch signaling inhibitor DAPT down-regulates cdk5 activity and modulates the distribution of neuronal cytoskeletal proteins: J. Kanungo, et al.; J. Neurochem. 106, 2236 (2008) 
  8. DAPT enhances the apoptosis of human tongue carcinoma cells: B.E. Grottklau, et al.; Int. J. Oral Sci. 1, 81 (2009) 
  9. Developmental expression of the slurp-like1/ly2.3/ly97.3 and slurp-like2/ly2.2/ly97.2 genes during zebrafish early embryogenesis: A. Kawahara, et al.; Gene Expr. Pattern 30, 32 (2018)
  10. 3D Bioprinted Human Cortical Neural Constructs Derived from Induced Pluripotent Stem Cells: F. Salaris, et al.; J. Clin. Med. 8, 1595 (2019)
  11. Regulation and role of glycophagy in skeletal muscle energy metabolism: T.D. Heden, et al.; Autophagy ahead of print (2021)
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