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AdipoGen Life Sciences
Suc-Leu-Tyr-AMC
45
CHF
CHF 45.00
In stock
AG-CP3-0017-M0055 mgCHF 45.00
| Product Details | |
|---|---|
| Synonyms | Suc-LY-AMC; Proteasome Substrate; Calpain Substrate; Papain Substrate |
| Product Type | Chemical |
| Properties | |
| Formula |
C29H33N3O8 |
| MW | 551.6 |
| Sequence |
N-Succinyl-Leu-Tyr-7-amido-4-methylcoumarin |
| CAS | 94367-20-1 |
| Purity Chemicals | >98% (TLC) |
| Appearance | Solid lyophilized powder. |
| Solubility | Soluble in DMSO or methanol. |
| Other Product Data |
Use: Add from DMSO stock directly to in vitro or in vivo assays at desired concentration. Typical concentrations range from 10-100µM. |
| InChi Key | RIYLNECMTVNMSO-GOTSBHOMSA-N |
| Smiles | CC(C)C[C@H](NC(=O)CCC(O)=O)C(=O)N[C@@H](CC1=CC=C(O)C=C1)C(=O)NC1=CC2=C(C=C1)C(C)=CC(=O)O2 |
| Shipping and Handling | |
| Shipping | AMBIENT |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice |
Avoid freeze/thaw cycles. Protect from light. |
| Use/Stability | Stable for at least 1 year after receipt when stored at -20°C. |
| Documents | |
| MSDS |
 Download PDF |
| Product Specification Sheet | |
| Datasheet |
Download PDF |
Description
- Fluorogenic substrate for measuring the chymotrypsin-like peptidase activity of the 20S proteasome.
- Fluorogenic substrate for calpain and papain.
- Peptide substrate for Ti protease from E.coli.
- Excitation: 360nm. Emission: 460nm.
- This substrate is useful for inhibitor screening and kinetic analysis.
Product References
- Comparative specificity and kinetic studies on porcine calpain I and calpain II with naturally occurring peptides and synthetic fluorogenic substrates: T. Sasaki, et al.; J. Biol. Chem. 259, 12489 (1984)
- Na+, K+-specific inhibition of protein and peptide hydrolyses by proteasomes from human hepatoma tissues: J.H. Seol, et al.; FEBS Lett. 247, 197 (1989)
- Protease Ti from Escherichia coli requires ATP hydrolysis for protein breakdown but not for hydrolysis of small peptides: K.M. Woo, et al.; J. Biol. Chem. 264, 2088 (1989)
- Ube2l3 gene expression is modulated by activation of the aryl hydrocarbon receptor: implications for p53 ubiquitination: O.D. Reyes-Hernandez, et al.; Biochem. Pharmacol. 80, 932 (2010)
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