Call +41-61-926-60-40 Fax +41-61-926-60-49

Phorbol 12-myristate 13-acetate

AG-CN2-0010-M001 1 mg INQ
AG-CN2-0010-M005 5 mg INQ
AG-CN2-0010-M010 10 mg INQ
AG-CN2-0010-M025 25 mg INQ
Actions
Select size:

* Required Fields

Amount:
Add to Cart
Call +41-61-926-60-40
Fax +41-61-926-60-49
Contact Us!

Additional Information

Product Data
Synonyms PMA; TPA; 12-O-Tetradecanoylphorbol 13-acetate
Properties
Formula C36H56O8
MW 616.8
Merck Index 14: 7332
CAS 16561-29-8
RTECS QH4377000
Source/Host Semisynthetic.
Purity ≥98% (HPLC)
Appearance White to off-white solid.
Solubility Soluble in DMSO, methanol, 100% ethanol, acetone, ether or dimethylformamide; almost insoluble in aqueous buffers.
Other Product Data Stock solutions are stable for up to 3 months at -20°C.
InChi Key PHEDXBVPIONUQT-RGYGYFBISA-N
Product Type Chemical
Shipping and Handling
Shipping AMBIENT
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice Protect from light.
Use/Stability Stable for at least 2 years after receipt when stored at -20°C.
Documents
MSDS Download Document Download

Product Description

  • Most commonly-used phorbol ester [1,2].
  • Binds to and activates protein kinase C (PKC) at nM concentrations [1,2,9]. 
  • Induces cell growth arrest through a variety of pathways including the mitogen-activated protein kinases (MAPKs), p38 and c-Jun N-terminal kinase (JNK) pathways mediated by cyclin dependent kinase (CDK) inhibitors such as p21WAF1/CIP1, p27KIP1, p15 and p16.
  • Potent mouse skin tumor promoter [3,4].
  • Promoter of inducible NOS (iNOS; NOS II) [5].
  • Apoptosis inducer [6].
  • Potential effective cancer therapeutic agent [7,8].
  • Inhibitor of anti-lipolytic activity of insulin [10].

Product References

  1. In vitro studies on the mode of action of the phorbol esters, potent tumor promoters: part 1: P.M. Blumberg; Crit. Rev. Toxicol. 8, 153 (1980) 
  2. In vitro studies on the mode of action of the phorbol esters, potent tumor promoters: part 2: P.M. Blumberg; Crit. Rev. Toxicol. 8, 199 (1981)
  3. Skin tumor promotion by phorbol esters is a two-stage process: G. Fürstenberger, et al.; PNAS 78, 7722 (1981)
  4. Phorbol esters as signal transducers and tumor promoters: M. Castagna; Biol. Cell 59, 3 (1987), Review
  5. Phorbol esters induce nitric oxide synthase activity in rat hepatocytes. Antagonism with the induction elicited by lipopolysaccharide: S. Hortelano, et al.; J. Biol. Chem. 267, 24937 (1992) 
  6. Phorbol ester induces apoptosis in HL-60 promyelocytic leukemia cells but not in HL-60 PET mutant: D.E. Macfarlane & P.S. O'Donnel; Leukemia 7, 1846 (1993) 
  7. Effect of intravenous infusions of 12-O-tetradecanoylphorbol-13- acetate (TPA) in patients with myelocytic leukemia: Preliminary studies on therapeutic efficacy and toxicity: T.H. Zheng, et al.; PNAS 95, 5357 (1998)
  8. 12-O-Tetradecanoylphorbol-13-acetate (TPA)-induced increase in depressed white blood cell counts in patients treated with cytotoxic cancer chemotherapeutic drugs: T.H. Zheng, et al.; PNAS 95, 5362 (1998)
  9. Characterization of phorbol esters activity on individual mammalian protein kinase C isoforms, using the yeast phenotypic assay: L. Saraiva, et al.; Eur. J. Pharmacol. 491, 101 (2004)
  10. Phorbol 12-myristate 13-acetate inhibits the antilipolytic action of insulin, probably via the activity of protein kinase Cε: J. Nakamura; Eur. J. Pharmacol. 648, 188 (2010)
No reviews for this product yet.