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AdipoGen Life Sciences
X-Gal
30
CHF
CHF 30.00
In stock
AG-CC1-0003-M05050 mgCHF 30.00
AG-CC1-0003-M250250 mgCHF 40.00
AG-CC1-0003-G0011 gCHF 55.00
AG-CC1-0003-G0055 gCHF 180.00
| Product Details | |
|---|---|
| Synonyms | 5-Bromo-4-chloro-3-indolyl β-D-galactoside |
| Product Type | Chemical |
| Properties | |
| Formula |
C14H15BrClNO6 |
| MW | 408.6 |
| CAS | 7240-90-6 |
| Purity Chemicals | ≥99% (HPLC) |
| Appearance | White to off-white powder. Clear, colourless to light yellow in solution. |
| Solubility | Soluble in dimethyl formamide. |
| Identity | Determined by IR. |
| Other Product Data |
Optical Rotation [α]D20 (c=1,DMF:water, 1:1): -61.5° ± 1.0° |
| InChi Key | OPIFSICVWOWJMJ-LMNFNBGQSA-N |
| Smiles | OCC1O[C@@H](OC2=CNC3=C2C(Cl)=C(Br)C=C3)C(O)[C@H](O)[C@H]1O |
| Shipping and Handling | |
| Shipping | AMBIENT |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice | Protect from light and moisture. |
| Use/Stability | Stable for at least 3 years after receipt when stored at -20°C. |
| Documents | |
| MSDS |
 Download PDF |
| Product Specification Sheet | |
| Datasheet |
Download PDF |
Description
- Molecular biology reagent.
- Chromogenic substrate for β-galactosidase.
- Used in gene cloning together with IPTG (Prod. No. AG-CC1-0002) to indicate whether a cell expresses the β-galactosidase enzyme, which is encoded by the lacZ gene, in a technique called blue/white screening.
- Distinguishes between recombinant and non-recombinant plasmids carrying the β-galactosidase gene.
- Used in two-hybrid analysis.
- X-Gal is used to determine E. coli and coliform content in drinking water samples.
Product References
- Chemiluminescent assay of various enzymes using indoxyl derivatives as substrate and its applications to enzyme immunoassay and DNA probe assay: H. Arakawa, et al.; Anal. Biochem. 199, 238 (1991)
- In situ histochemical detection of beta-galactosidase activity in lung: assessment of X-Gal reagent in distinguishing lacZ gene expression and endogenous beta-galactosidase activity: D.J. Weiss, et al.; Hum. Gene Ther. 8, 1545 (1997)
- A novel colorimetric yeast bioassay for detecting trichothecene mycotoxins: K.H. Engler, et al.; J. Microbiol. Methods 35, 207 (1999)
- Molecular Cloning: A Laboratory Manual 3rd Edition: J. Sambrook & D. Russell; Cold Spring Harbor Laboratory Press (2001)
- Effects of tissue pressure on transgene expression characteristics via renal local administration routes from ureter or renal artery in the rat kidney: N. Oyama, et al.; MDPI Pharmaceutics 12, 114 (2020)
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