Additional Information
| Product Data | |
|---|---|
| Synonyms | Interleukin-1β Convertase; IL-1BC; Interleukin-1β-converting Enzyme; ICE |
| Properties | |
| Application Set | Quantitative ELISA |
| Specificity | Detects mouse Caspase-1 (p10 and p20 domain). Does not detect human Caspase-1. |
| Crossreactivity | Mouse |
| Quantity | Contains sufficient materials to run ELISAs on 5 x 96-well plates. |
| Sensitivity | 50pg/ml |
| Range | 0.15ng/ml to 10ng/ml. |
| Sample Type | Cell Culture Supernatant |
| Assay Type | Sandwich |
| Detection Type | Colorimetric |
| Kit Contains | 1 vial standard protein (1μg) (lyophilized) [STD] 1 vial coating antibody (120μg) [COAT] 1 vial detection antibody (60μg) [DET] 1 vial streptavidin-HRP (10μg) (lyophilized) [STREP] |
| Product Type | Kit |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Short Term Storage | -20°C |
| Long Term Storage | -20°C |
| Handling Advice | Avoid freeze/thaw cycles. |
| Documents | |
| Manual | 
Download |
| MSDS |
Download |
Product Description
Caspase-1 is the best-described inflammatory caspase. It processes the cytokines interleukin-1β (IL-1β) and interleukin-18 (IL-18) and induces pyroptotic cell death. Caspase-1 is activated by multiprotein complexes called inflammasomes in response to numerous stimuli that are detected through distinct inflammasomes. NLRC4 responds to cytosolic flagellin, murine NLRP1b responds to anthrax lethal toxin, AIM2 responds to cytosolic DNA and NLRP3/NALP3 responds to a variety of agonists including crystals.
The caspase-1 (mouse) Matched Pair Detection Set contains all reagents for the development of a sandwich ELISA to measure caspase-1 (mouse) in cell culture supernatants. This Matched Pair Detection Set is a quantitative detection method, alternative to Western blotting to measure caspase-1 secretion.
Mouse Caspase-1 is detected in cell culture supernatants by ELISA using Caspase-1 (mouse) Matched Pair Detection Set (Prod. No AG-46B-0003).
Method: Caspase-1 (mouse) was measured by ELISA in supernatants of differentiated bone marrow-derived dendritic cells (BMDCs) from wild-type (WT) or caspase-1-/- (KO) mice activated or not by 5 μM Nigericin (Nig) (AG-CN2-0020) for 30 min.
Method: Caspase-1 (mouse) was measured by ELISA in supernatants of differentiated bone marrow-derived dendritic cells (BMDCs) from wild-type (WT) or caspase-1-/- (KO) mice activated or not by 5 μM Nigericin (Nig) (AG-CN2-0020) for 30 min.
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