AdipoGen Life Sciences

VEGF 165 (human) (rec.)

CHF 180.00
In stock
AG-40T-0043-C0055 µgCHF 180.00
AG-40T-0043-C02020 µgCHF 420.00
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Product Details
Synonyms Vascular Endothelial Growth Factor; VEGF-A; VPF
Product Type Protein
Properties
Source/Host E. coli
Sequence Human VEGF 165 (165aa).
Crossreactivity Human
Biological Activity The ED50 for stimulation of cell proliferation in human umbilical vein endothelial cells by VEGF 165 has been determined to be in the range of 1-5ng/ml.
MW ~38.2kDa
Purity ≥95% (SDS-PAGE)
Endotoxin Content <0.1ng/μg protein.
Reconstitution Reconstitute with 50mM acetic acid to a concentration not lower than 50 μg/ml.
Formulation Lyophilized.
Other Product Data UniProt link P15692-4: VEGF 165 (human)
Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice After reconstitution, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.
Centrifuge lyophilized vial before opening and reconstitution.
For long term storage we recommend to add at least 0.1% human or bovine serum albumin.
Use/Stability Stable for at least 6 months after receipt when stored at -20°C.
Documents
MSDS Download PDF
Product Specification Sheet
Datasheet Download PDF
Description

Human vascular endothelial growth factor (VEGF 165) is produced as a homodimer. It binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. VEGF 165 is a specific mitogen for vascular endothelial cells and a strong angiogenic factor in vivo. In addition to its action as a mitogen it is a potent vascular permeability factor (VPF) in vivo. It is also a chemoattractant molecule for monocytes and endothelial cells. It induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels.

Product References

Developmental coronary maturation is disturbed by aberrant cardiac vascular endothelial growth factor expression and Notch signalling: N.M.S. van den Akker, et al.; Cardiovasc. Res. 78, 366 (2008)

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