Call +41-61-926-60-40 Fax +41-61-926-60-49

Slit2 (C fragment) (human) (rec.)

AG-40B-0168-C010 10 µg INQ
AG-40B-0168-3010 3 x 10 µg INQ
Actions
Select size:

* Required Fields

Amount:
Add to Cart
Call +41-61-926-60-40
Fax +41-61-926-60-49
Contact Us!

Additional Information

Product Data
Synonyms Slit Homolog 2 Protein (C fragment)
Properties
Source/Host HEK 293 cells
Sequence Human Slit2 (aa 1122-1529) is fused at the C-terminus to a FLAG®-tag.
Crossreactivity Human
Biological Activity Activates an UCP1-luciferase construct in mouse fat primary cells at a concentration of 20nM.
MW ~55kDa
Purity ≥95% (SDS-PAGE)
Endotoxin Content <0.01EU/μg purified protein (LAL test; Lonza).
Concentration 0.1mg/ml after reconstitution.
Reconstitution Reconstitute with 100μl sterile water.
Formulation Lyophilized. Contains PBS.
Other Product Data Uni-Prot link 0904813: Slit2 (C fragment)
Product Type Protein
Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. PBS containing at least 0.1% BSA should be used for further dilutions.
Use/Stability Stable for at least 6 months after receipt when stored at -20°C.
Documents
MSDS Download Document Download

Product Description

The Slits are secreted proteins that bind to Robo receptors and play a role in axon guidance and neuronal migration. The Slit family in mice and humans comprises three members: Slit1, Slit2 and Slit3. These are all extracellular matrix proteins of 180-200kDa. In vivo, Slit2 is cleaved into 140kDa N-terminal (Slit2-N) and 55-60 kDa C-terminal (Slit2-C) fragments. Only Slit2-N and Slit2 full-length bind the Robo proteins. Recently, it has been shown that Slit2 is highly expressed in adipose tissues under the control of PRDM16 and cold exposure. Slit2 is posttranslationally cleaved by adipocytes and the resulting Slit2-C fragment improves glucose homeostasis in mice by activating a thermogenic PKA/cAMP pathway not mediated by β-adrenergic receptors in adipocytes.
No reviews for this product yet.