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AdipoGen Life Sciences
COMP (rat):Angiopoietin-1 (human) (rec.)
250
CHF
CHF 250.00
In stock
AG-40B-0147-C01010 µgCHF 250.00
AG-40B-0147-C05050 µgCHF 840.00
AG-40B-0147-50105 x 10 µgCHF 850.00
BULK available!
Figure 1: COMP (rat):Angiopoietin-1 (human) (rec.) (AG-40B-0147) binds to Tie-2 (human):Fc (human). Method: Tie-2 (human):Fc was coated on an ELISA plate at 1μg/ml. After blocking and washing steps, indicated concentrations of COMP (rat):Angiopoietin-1 (human) (AG-40B-0147) or a Control were added. Following incubation for 1 hour at RT, the binding was detected using an anti-FLAG antibody (HRP).
| Product Details | |
|---|---|
| Synonyms | COMP-Ang1; COMP:Ang-1; COMP Angiopoietin 1; COMP ANGPT1 |
| Product Type | Protein |
| Properties | |
| Source/Host | CHO cells |
| Sequence |
The fibrinogen-like domain (aa 284-498) of human angiopoietin-1 is fused at the N-terminus to the coiled-coil domain of rat COMP (cartilage oligomeric matrix protein) and to a FLAG®-tag. |
| Crossreactivity | Human |
| Specificity |
Binds to human angiopoietin-1 receptor (Tie-2). |
| Biological Activity |
More potent than native angiopoietin-1. The protein forms pentameric structures. |
| MW | 35kDa (SDS-PAGE) |
| Purity | ≥95% (SDS-PAGE) |
| Endotoxin Content | <0.05EU/μg purified protein (LAL test). |
| Concentration |
After reconstitution: for 10µg size: 0.1mg/ml for 50µg size: 1 mg/ml |
| Reconstitution |
10µg size: Reconstitute with 100µl sterile water. 50µg size: Reconstitute with 50µl sterile water. |
| Formulation | Lyophilized. Contains PBS + 0.02% CHAPS. |
| Other Product Data |
UniProt link Q15389: Angiopoietin-1 (human) |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice |
After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution. PBS containing at least 0.1% BSA should be used for further dilutions. |
| Use/Stability |
Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C. |
| Documents | |
| MSDS |
 Download PDF |
| Product Specification Sheet | |
| Datasheet |
Download PDF |
Description
- Soluble, stable and more potent angiopoietin-1 (Ang-1) variant.
Product References
- COMP-Ang1: a designed angiopoietin-1 variant with nonleaky angiogenic activity: CH. Cho, et al.; PNAS 101, 5547 (2004)
- COMP-angiopoietin-1 promotes wound healing through enhanced angiogenesis, lymphangiogenesis, and blood flow in a diabetic mouse model: CH. Cho, et al.; PNAS 103, 4946 (2006)
- Protective role of COMP-Ang1 in ischemic rat brain: HY. Shin, et al.; J. Neurosci. Res. 88, 1052 (2010)
- COMP-angiopoietin-1 promotes cavernous angiogenesis in a type 2 diabetic rat model: SO. Kim, et al.; J. Korean Med. Sci. 28, 725 (2013)
- COMP-Ang1 accelerates chondrocyte maturation by decreasing HO-1 expression: S. Kim, et al.; J. Cell. Biochem. 114, 2513 (2013)
- EphrinB2/EphB4 signaling regulates non‐sprouting angiogenesis by VEGF: E. Groppa, et al.; EMBO Rep. e45054 (2018)
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