AdipoGen Life Sciences

Fc (human):FasL, Soluble (human) (rec.)

CHF 335.00
In stock
AG-40B-0132-C01010 µgCHF 335.00
AG-40B-0132-30103 x 10 µgCHF 670.00
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Product Details
Synonyms APO-1L; CD95L; CD178; TNFSF6; Fas Ligand
Product Type Protein
Properties
Source/Host HEK 293 cells
Sequence

Human FasL (aa 139-281) is fused at the N-terminus to the Fc portion of human IgG1.

Crossreactivity Human
Mouse
Specificity

Binds to human and mouse Fas.

Biological Activity

Induces apoptosis of human Jurkat T cells at a concentration of <0.2ng/ml.

MW ~50kDa (SDS-PAGE)
Purity ≥95% (SDS-PAGE)
Endotoxin Content <0.01EU/μg purified protein (LAL test).
Concentration 0.1mg/ml after reconstitution.
Reconstitution Reconstitute with 100μl sterile water.
Formulation Lyophilized. Contains PBS.
Protein Negative Control

Fc (human) IgG1 Control (rec.)

Other Product Data

UniProt link P48023: FasL (human)

FLAG is a registered trademark of Sigma-Aldrich Co.

Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice After reconstitution, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.
Centrifuge lyophilized vial before opening and reconstitution.
PBS containing at least 0.1% BSA should be used for further dilutions.
Use/Stability Stable for at least 6 months after receipt when stored at -20°C.
Working aliquots are stable for up to 3 months when stored at -20°C.
Documents
MSDS Download PDF
Product Specification Sheet
Datasheet Download PDF
Description

Fc (human):FasL, Soluble (human) is a high activity construct in which two trimeric FasL are artificially linked via the Fc binding domain of human IgG1. This construct very effectively simulates the natural membrane-assisted aggregation of FasL in vivo. FasL is a cytokine that binds to TNFRSF6/Fas, a receptor that transduces the apoptotic signal into cells. It is involved in cytotoxic T cell mediated apoptosis and in T cell development.

Product References
  1. Two adjacent trimeric Fas ligands are required for Fas signaling and formation of a death-inducing signaling complex: N. Holler, et al.; Mol. Cell. Biol. 23, 1428 (2003)
  2. Immune response to intravenous immunoglobulin in patients with Kawasaki disease and MIS-C: Y.P. Zhu, et al.; J. Clin. Invest. 131, e147076 (2021)
  3. NET formation is a default epigenetic program controlled by PAD4 in apoptotic neutrophils: Y.P. Zhu, et al.; Sci. Adv. 9, 1397 (2023)
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