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AdipoGen Life Sciences
Angiopoietin-2 (human) (rec.)
240
CHF
CHF 240.00
In stock
AG-40B-0114-C01010 µgCHF 240.00
AG-40B-0114-30103 x 10 µgCHF 480.00
Figure 1: Binding of Angiopoietin-2 (human) (rec.) (Prod. No. AG-40B-0114) to its receptor Tie-2 (h) by ELISA. Methods: Tie-2 (human):Fc (h) was coated on an ELISA plate at 1µg/ml. After blocking and washing steps, indicated concentrations of Angiopoietin-2 (human) (rec.) of different lots were added. Following incubation for 1 h at RT, the binding was detected using an anti-FLAGantibody (HRP).
| Product Details | |
|---|---|
| Synonyms | Ang-2; Ang2; Angpt2 |
| Product Type | Protein |
| Properties | |
| Source/Host | HEK 293 cells |
| Sequence | Human angiopoietin-2 (aa 19-496) is fused at the N-terminus to a FLAG®-tag. |
| Crossreactivity |
Human Mouse |
| Specificity | Binds to the human and mouse Tie-2 receptor. |
| MW | ~70kDa (SDS-PAGE) |
| Purity | ≥95% (SDS-PAGE) |
| Endotoxin Content | <0.01EU/μg purified protein (LAL test; Lonza). |
| Concentration | 0.1 mg /ml after reconstitution. |
| Reconstitution | Reconstitute with 100μl sterile water. |
| Formulation | Lyophilized. Contains PBS with trehalose and Brij35. |
| Other Product Data |
UniProt link O15123: Angiopoietin-2 (human) FLAG is a registered trademark of Sigma-Aldrich Co. |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice |
After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution. PBS containing at least 0.1% BSA should be used for further dilutions. |
| Use/Stability |
Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C. |
| Documents | |
| MSDS |
 Download PDF |
| Product Specification Sheet | |
| Datasheet |
Download PDF |
Description
Angiopoietin-1 (Ang-1) and Angiopoietin-2 (Ang-2) are closely related secreted ligands which bind with similar affinity to Tie-2. Tie-2 and angiopoietins have been shown to play critical roles in embryogenic angiogenesis and in maintaining the integrity of the adult vasculature. Ang-1 activates Tie-2 signaling on endothelial cells to promote chemotaxis, cell survival, cell sprouting, vessel growth and stabilization. Ang-2 has been identified as a secreted protein ligand of Tie-2 and has alternatively been reported to be an antagonist for Ang-1 induced Tie-2 signaling as well as an agonist for Tie-2 signaling, depending on the cell context.
Product References
- Interaction between Tie receptors modulates angiogenic activity of angiopoietin2 in endothelial progenitor cells: K.L. Kim, et al.; Cardiovasc. Res. 72, 394 (2006)
- Two natural eudesmane-type sesquiterpenes from Laggera alata inhibit angiogenesis and suppress breast cancer cell migration through VEGF- and Angiopoietin 2-mediated signaling pathways: N. Liang, et al.; Int. J. Oncol. 51, 213 (2017)
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