AdipoGen Life Sciences

Netrin-1 (human):Fc (human) (rec.)

CHF 350.00
In stock
AG-40B-0075-C01010 µgCHF 350.00
AG-40B-0075-30103 x 10 µgCHF 695.00
AG-40B-0075-C100100 µgCHF 1'560.00
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Product Details
Synonyms NTN1
Product Type Protein
Properties
Source/Host HEK 293 cells
Sequence

Human netrin-1 (aa 25-604) is fused at the C-terminus to the Fc portion of human IgG1.

Crossreactivity Human
Mouse
Rat
Specificity

Binds to human, mouse and rat UNC5B.

Biological Activity

Induces pluripotent stem cell (iPS) formation and improves reprogramming efficiency of human and mouse somatic cells by inhibiting apoptosis mediated by the receptors DCC or UNC5B (at 150ng /ml). Induces axon outgrowth.

MW ~90kDa (SDS-PAGE)
Purity ≥95% (SDS-PAGE)
Endotoxin Content <0.1EU/μg purified protein (LAL test; Lonza).
Concentration 0.1mg/ml after reconstitution.
Reconstitution Reconstitute with 100μl sterile distilled water.
Formulation Lyophilized. Contains PBS plus protein stabilizer. No BSA included. The protein stabilizer shows no interference with protein activity.
Protein Negative Control

Fc (human) IgG1 Control (rec.)

Other Product Data

UniProt link O95631: Netrin-1 (human)

Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice After reconstitution, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.
Centrifuge lyophilized vial before opening and reconstitution.
PBS containing at least 0.1% BSA should be used for further dilutions.
Use/Stability Stable for at least 6 months after receipt when stored at -20°C.
Working aliquots are stable for up to 3 months when stored at -20°C.
Documents
MSDS Download PDF
Product Specification Sheet
Datasheet Download PDF
Description

Netrin-1 controls guidance of CNS commissural axons and peripheral motor axons. Its association with either DCC or some UNC5 receptors will lead to axon attraction or repulsion, respectively. It also serve as a survival factor via its association with its receptors which prevent the initiation of apoptosis. Netrin-1 is also Involved in tumorigenesis by regulating apoptosis. Netrin-1 promotes atherosclerosis by retaining macrophages in the artery wall. Netrin-1 also governs induced pluripotent stem cell (iPS) formation and improves reprogramming efficiency of human and mouse somatic cells by limiting apoptosis mediated by Netrin-1 receptors DCC or UNC5b.

Product References
  1. DCC constrains tumour progression via its dependence receptor activity: M. Castets, et al.; Nature 482, 534 (2011)
  2. The amyloid precursor protein regulates netrin-1 mediated commissural axon outgrowth: N. Rama, et al.; J. Biol. Chem. 287, 30014 (2012)
  3. Signaling switch of the axon guidance receptor Robo3 during vertebrate evolution: P. Zelina, et al.; Neuron 84, 1258 (2014)
  4. Netrin-1 regulates somatic cell reprogramming and pluripotency maintenance: D. Ozmadenci, et al.; Nat. Commun. 6, ID7398 (2015)
  5. Development of Targeted Microbubbles for Ultrasound Molecular Imaging of Breast Cancer: J. Wischhusen, et al.; RITS 150 (2015)
  6. Structural Decoding of the Netrin-1/UNC5 Interaction and its Therapeutical Implications in Cancers: M. Grandin, et al.; Cancer Cell 29, 173 (2016)
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