AdipoGen Life Sciences

DLL4 (mouse):Fc (human) (rec.)

CHF 130.00
In stock
AG-40A-0145-C01010 µgCHF 130.00
AG-40A-0145-C05050 µgCHF 290.00
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Product Details
Synonyms Delta-like Protein 4; Delta4
Product Type Protein
Properties
Source/Host HEK 293 cells
Sequence

Signal peptide and extracellular domain of mouse DLL4 (aa 1-532) is fused at the C-terminus to the Fc portion of human IgG1.

Crossreactivity Mouse
Biological Activity

Inhibits adipogenesis of 3T3L-1 cells.

MW ~95kDa (SDS-PAGE)
Purity ≥90% (SDS-PAGE)
Endotoxin Content <0.1EU/μg purified protein (LAL test; Lonza).
Formulation Liquid. 0.2μm-filtered solution in PBS.
Protein Negative Control

Fc (human) IgG1 Control (rec.)

Other Product Data

UniProt link Q9JI71: DLL4 (mouse) [Precursor]

Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice After opening, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.
For maximum product recovery after thawing, centrifuge the vial before opening the cap.
Use/Stability Stable for at least 6 months after receipt when stored at -20°C.
Working aliquots are stable for up to 3 months when stored at -20°C.
Documents
MSDS Download PDF
Product Specification Sheet
Datasheet Download PDF
Description

The Notch ligand delta-like protein 4 (DLL4) is expressed highly and selectively within the arterial endothelium and has been shown to function as a ligand for Notch1 and Notch4. It is induced by VEGF as a negative feedback regulator and acts to prevent overexuberant angiogenic sprouting, pro-moting the timely formation of a well differentiated vascular network. DLL4-Notch1 signaling regulates the formation of appropriate numbers of tip cells to control vessel sprouting and branching in the mouse retina.

Product References
  1. Endothelial Wnt/β-catenin signaling inhibits glioma angiogenesis and normalizes tumor blood vessels by inducing PDGF-B expression: M. Reis, et al.; J. Exp. Med. 209, 1611 (2012)
  2. Flt-1 (vascular endothelial growth factor receptor-1) is essential for the vascular endothelial growth factor-Notch feedback loop during angiogenesis: J.C. Chappell, et al.; Arterioscler. Thromb. Vasc. Biol. 33, 1952 (2013)
  3. Combinatorial microenvironmental regulation of liver progenitor differentiation by Notch ligands, TGFβ, and extracellular matrix: K.B. Kaylan, et al.; Scientific Rep. 6, 23490 (2016)
  4. Spatial patterning of liver progenitor cell differentiation mediated by cellular contractility and Notch signaling: K.B. Kaylan, et al.; Elife 7, e38536 (2018)
  5. Reciprocal communication between astrocytes and endothelial cells is required for astrocytic glutamate transporter 1 (GLT-1) expression: Z. Martinez-Lozada & M.B. Robinson; Neurochem. 139, 104787 (2020)
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