AdipoGen Life Sciences

FGF-23 (human) (rec.) (His)

CHF 230.00
In stock
AG-40A-0114Y-C01010 µgCHF 230.00
More Information
Product Details
Synonyms Fibroblast Growth Factor 23; Tumor-derived Hypophosphatemia-inducing Factor
Product Type Protein
Properties
Source/Host HEK 293 cells
Sequence Signal peptide and human FGF-23 (aa 1-251) are fused at the C-terminus to a His-tag.
Crossreactivity Human
Biological Activity Activates ERK and FRS2α phosphorylation in Klotho expressing cells.
MW ~30kDa (SDS-PAGE, full-length). FGF23 is cleaved between Arg179 and Ser180 (two bands of ~14kDa and ~16kDa).
Purity ≥90% (SDS-PAGE)
Endotoxin Content <0.01EU/μg purified protein (LAL test; Lonza).
Concentration 0.1mg/ml after reconstitution.
Reconstitution Reconstitute with 100μl sterile water.
Formulation Lyophilized. Contains PBS.
Other Product Data UniProt link Q9GZV9: FGF-23 (human)
Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice After reconstitution, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.
Centrifuge lyophilized vial before opening and reconstitution.
PBS containing at least 0.1% BSA should be used for further dilutions.
Use/Stability Stable for at least 6 months after receipt when stored at -20°C.
Working aliquots are stable for up to 3 months when stored at -20°C.
Documents
MSDS Download PDF
Product Specification Sheet
Datasheet Download PDF
Description

FGF-23 (Fibroblast growth factor 23) is a regulator of phosphate homeostasis. It upregulates EGR1 expression in the presence of KLBy. Acts directly on the parathyroid to decrease PTH secretion. Regulates the vitamin-D metabolism. Negatively regulates osteoblast differentiation and matrix mineralization. Defects in FGF-23 are the cause of autosomal dominant hypophosphataemic rickets (ADHR) and of hyperphosphatemic familial tumoral calcinosis (HFTC).

Product References
  1. Maternally-derived antibody to fibroblast growth factor-23 reduced dietary phosphate requirements in growing chicks: E.A. Bobeck, et al.; BBRC 420, 666 (2012)
  2. Antibody to fibroblast growth factor 23-peptide reduces excreta phosphorus of laying hens: Z. Ren, et al.; Poultry Science 96, 127 (2017)
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