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AdipoGen Life Sciences
FGF-23 (human):Fc (human) (rec.)
205
CHF
CHF 205.00
In stock
AG-40A-0109-C01010 µgCHF 205.00
AG-40A-0109-C05050 µgCHF 430.00
Figure 1: ERK and FRS2α phosphorylation induced by FGF-21 in Klotho expressing cells.
Klotho expressing HEK 293EBNA cells were serum starved for 16hr and then stimulated with hFGF-23-His, FGF-23-Fc (Prod. No. AG-40A-0109), mCD137-Fc (Fc control) and FGF-b (positive control) for 10 min, respectively. Antibodies against pFRS2α, pERK1/2 & total ERK1/2 were used for immunoblotting.
Lane 1: Mock (non-treated)
Lane 2: Mock + hFGF23-Fc 1μg/ml
Lane 3: Mock + hFGF23-His 1μg/ml
Lane 4: Klotho + hFGF23-Fc 1μg/ml
Lane 5: Klotho + hFGF23-Fc 4μg/ml
Lane 6: Klotho + mCD137-Fc 1μg/ml
Lane 7: Klotho (non-treated)
Lane 8: Klotho + 100ng/ml FGF-b
Klotho expressing HEK 293EBNA cells were serum starved for 16hr and then stimulated with hFGF-23-His, FGF-23-Fc (Prod. No. AG-40A-0109), mCD137-Fc (Fc control) and FGF-b (positive control) for 10 min, respectively. Antibodies against pFRS2α, pERK1/2 & total ERK1/2 were used for immunoblotting.
Lane 1: Mock (non-treated)
Lane 2: Mock + hFGF23-Fc 1μg/ml
Lane 3: Mock + hFGF23-His 1μg/ml
Lane 4: Klotho + hFGF23-Fc 1μg/ml
Lane 5: Klotho + hFGF23-Fc 4μg/ml
Lane 6: Klotho + mCD137-Fc 1μg/ml
Lane 7: Klotho (non-treated)
Lane 8: Klotho + 100ng/ml FGF-b
| Product Details | |
|---|---|
| Synonyms | Fibroblast Growth Factor 23; Tumor-derived Hypophosphatemia-inducing Factor |
| Product Type | Protein |
| Properties | |
| Source/Host | HEK 293 cells |
| Sequence |
Human FGF-23 (aa25-251) are fused at the C-terminus to the Fc portion of human IgG1. |
| Crossreactivity | Human |
| Biological Activity |
Activates ERK and FRS2α phosphorylation in Klotho expressing cells. |
| MW | ~60kDa (SDS-PAGE, full-length); FGF23 is cleaved between arg179 and ser 180 (~40kDa) |
| Purity | ≥90% (SDS-PAGE) |
| Endotoxin Content | <0.1EU/μg purified protein (LAL test; Lonza). |
| Concentration | 1mg/ml |
| Formulation | Liquid. 0.2μm-filtered solution in PBS. |
| Protein Negative Control | |
| Other Product Data |
UniProt link Q9GZV9: FGF-23 (human) |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice |
After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. For maximum product recovery after thawing, centrifuge the vial before opening the cap. |
| Use/Stability |
Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C. |
| Documents | |
| MSDS |
 Download PDF |
| Product Specification Sheet | |
| Datasheet |
Download PDF |
Description
FGF-23 (Fibroblast growth factor 23) is a regulator of phosphate homeostasis. It upregulates EGR1 expression in the presence of KLBy. Acts directly on the parathyroid to decrease PTH secretion. Regulates the vitamin-D metabolism. Negatively regulates osteoblast differentiation and matrix mineralization. Defects in FGF-23 are the cause of autosomal dominant hypophosphataemic rickets (ADHR) and of hyperphosphatemic familial tumoral calcinosis (HFTC).
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