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AdipoGen Life Sciences
Jagged-1 (human):Fc (human) (rec.)
235
CHF
CHF 235.00
In stock
AG-40A-0081-C01010 µgCHF 235.00
AG-40A-0081-C05050 µgCHF 430.00
Figure 1: Induction of IL-6 expression in human dermal fibroblasts by Jagged-1 (human):Fc (human) (rec.) (Prod. No. AG-40A-0081). Method: Jagged-1 (human):Fc was coated on a 12-well plate at 1µg/ml overnight at 4°C. Human dermal fibroblasts were cultured in the presence or absence of Jagged-1 (human):Fc for 72 hours. Real time quantitative PCR was used to quantify the expression of IL-6. Picture courtesy of the lab of Prof. Gian-Paolo Dotto, Department of Biochemistry, University of Lausanne
Figure 2: Induction of Hes-1 with the treatment of recombinant human Jagged1-Fc (Prod. No. AG-40A-0081).
A mouse preadpipocyte cell line, 3T3L1, was stimulated with 5μg/ml of human Jagged1-Fc as in indicated time points and each cell lysate was prepared and subjected to western blot by using anti-human Hes1 or GAPDH.
A mouse preadpipocyte cell line, 3T3L1, was stimulated with 5μg/ml of human Jagged1-Fc as in indicated time points and each cell lysate was prepared and subjected to western blot by using anti-human Hes1 or GAPDH.
Figure 3: Adipogenesis inhibition of 3T3L-1 cells.
3T3L1 cells were maintained in DMEM supplemented with 10% FBS and penicillin-streptomycin. When the cells were confluently grown adipogenesis was initiated by adding IBMX, Dexamethasone, and insulin to 0.5mM, 1μM, and 10μg/ml, respectively and continued for 2 days (day 0). The medium was replaced every 2 days with new medium containing insulin in the presence or absence of 5μg/ml of human Jagged1-Fc (Prod. No. AG-40A-0081) and human CD137-Fc as a control-Fc. Staining with Oil Red O was typically performed on day 7.
3T3L1 cells were maintained in DMEM supplemented with 10% FBS and penicillin-streptomycin. When the cells were confluently grown adipogenesis was initiated by adding IBMX, Dexamethasone, and insulin to 0.5mM, 1μM, and 10μg/ml, respectively and continued for 2 days (day 0). The medium was replaced every 2 days with new medium containing insulin in the presence or absence of 5μg/ml of human Jagged1-Fc (Prod. No. AG-40A-0081) and human CD137-Fc as a control-Fc. Staining with Oil Red O was typically performed on day 7.
Figure 4: Adipogenesis inhibition. Macroscopic pictures.
| Product Details | |
|---|---|
| Synonyms | Protein Jagged-1; hJ1; CD339; JAG1 |
| Product Type | Protein |
| Properties | |
| Source/Host | HEK 293 cells |
| Sequence |
Signal peptide and extracellular domain of human Jagged-1 (HJ1; CD339) (aa 1-1067) are fused at the C-terminus to the Fc portion of human IgG1. |
| Crossreactivity | Human |
| Biological Activity |
Inhibits adipogenesis of mesenchymal stem cells (MSCs). Induces Hes-1 in 3T3L-1 cells. |
| MW | ~150kDa (SDS-PAGE) |
| Purity | ≥90% (SDS-PAGE) |
| Endotoxin Content | <0.1EU/μg purified protein (LAL test; Lonza). |
| Concentration | 0.5mg/ml |
| Formulation | Liquid. 0.2μm-filtered solution in PBS. |
| Protein Negative Control | |
| Other Product Data |
Uniprot link P78504: Jagged-1 [Precursor] |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice |
After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. For maximum product recovery after thawing, centrifuge the vial before opening the cap. |
| Use/Stability |
Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C. |
| Documents | |
| MSDS |
 Download PDF |
| Product Specification Sheet | |
| Datasheet |
Download PDF |
Description
Jagged-1 is a ligand for multiple Notch receptors and involved in the mediation of Notch signaling. It is involved in cell-fate decisions during hematopoiesis. Is involved in early and late stages of mammalian cardiovascular development. Inhibits myoblast differentiation. Enhances fibroblast growth factor-induced angiogenesis (in vitro). Defects in Jagged-1 are the cause of Alagille syndrome type 1 (ALGS1) and tetralogy of Fallot (TOF).
Product References
- Jagged1 heterozygosity in mice results in a congenital cholangiopathy which is reversed by concomitant deletion of one copy of Poglut1 (Rumi): S.M. Thakurdas, et al.; Hepatology 63, 550 (2016)
- Single factors alone can induce mesenchymal-like morphology, but not promote full EMT in breast cancer cell lines with different hormone statuses: M.K. Tveitaras, et al.; Exp. Cell Res. 359, 257 (2017)
- Notch ligands regulate the muscle stem-like state ex vivo but are not sufficient for retaining regenerative capacity: H. Sakai, et al.; PLoS ONE 12, e0177516 (2017)
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