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AdipoGen Life Sciences
ANGPTL4 (mouse) (rec.)
190
CHF
CHF 190.00
In stock
AG-40A-0075-C01010 µgCHF 190.00
AG-40A-0075-C05050 µgCHF 380.00
Figure 1: ERK phosphorylation induced by hANGPTL4 in THP-1 cells.
THP-1 monocyte cells were serum starved for 16hr and then stimulated with ANGPTL4 (mouse) (rec.) (Prod. No. AG-40A-0075) (500ng/ml) for 1, 2 and 3 hrs, respectively. Antibodies against pERK1/2 & total ERK1/2 were used for immunoblotting.
THP-1 monocyte cells were serum starved for 16hr and then stimulated with ANGPTL4 (mouse) (rec.) (Prod. No. AG-40A-0075) (500ng/ml) for 1, 2 and 3 hrs, respectively. Antibodies against pERK1/2 & total ERK1/2 were used for immunoblotting.
| Product Details | |
|---|---|
| Synonyms | Angiopoietin-like Protein 4; FIAF; Fasting-induced Adipose Factor; HFARP; Hepatic Fibrinogen/Angiopoietin-related Protein |
| Product Type | Protein |
| Properties | |
| Source/Host | COS-7 cells |
| Sequence |
Mouse ANGPTL4 (aa 1-410) is fused at the C-terminus to a FLAG®-tag. |
| Crossreactivity | Mouse |
| Biological Activity |
Activates ERK phosphorylation in THP-1 cells. |
| MW | ~50kDa (SDS-PAGE) |
| Purity | ≥90% (SDS-PAGE) |
| Endotoxin Content | <0.1EU/μg purified protein (LAL test; Lonza). |
| Concentration | 0.5mg/ml |
| Formulation | Liquid. 0.2μm-filtered solution in PBS. |
| Other Product Data |
UniProt link Q9Z1P8: ANGPTL4 (mouse) [Precursor] FLAG is a registered trademark of Sigma-Aldrich Co. |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice |
After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. For maximum product recovery after thawing, centrifuge the vial before opening the cap. |
| Use/Stability |
Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C. |
| Documents | |
| MSDS |
 Download PDF |
| Product Specification Sheet | |
| Datasheet |
Download PDF |
Description
ANGPTL4 (Angiopoietin-like protein 4) mainly expressed in endothelial cells (hypoxia-induced). Regulates angiogenesis and modulates tumorigenesis and directly regulates lipid, glucose, and energy metabolism. Inhibits proliferation, migration, and tubule formation of endothelial cells and reduces vascular leakage. ANGPTL4 is a protein consisting of an N-terminal coiled-coil domain and a C-terminal fibrinogen-like domain (FLD). Both domains have distinct biological functions. The coiled-coil domain is responsible for the inhibitory effects on lipoprotein lipase (LPL) converting the active form of LPL into an inactive form, and the FLD domain mediates its antiangiogenic functions. The coiled coil and the FLD domains are separated by a short linker that can be cleaved after secretion. ANGPTL4 appears on the cell surface as the full-length form, where it can be released by heparin treatment. ANGPTL4 protein is then proteolytically cleaved by proprotein convertases (PCs), including furin, PC5/6, paired basic amino acid-cleaving enzyme 4, and PC7.
Product References
- Regulation of energy balance by the hypothalamic lipoprotein lipase regulator Angptl3: H.K. Kim, et al.; Diabetes 64, 1142 (2015)
- Angiopoietin-like peptide 4 regulates insulin secretion and islet morphology: H.K. Kim, et al.; BBRC 485, 113 (2017)
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