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AdipoGen Life Sciences
ST2 (human):Fc (human) (rec.)
505
CHF
CHF 505.00
In stock
AG-40A-0059-C05050 µgCHF 505.00
Figure 1: hIL-33 binding to hST2-Fc confirmed by pull down assay.
a) pull down of h-IL-33 by hST2-Fc.
b) pull down of hST2-Fc by h-IL-33-His.
5 μg of hST2-Fc (Prod. No. AG-40A-0059) (or control Fc protein), 2 μg of hIL-33-His (or control His protein), and protein G resin (or anti-His resin) were incubated in 0.5ml RIPA buffer overnight at 4°C. The precipitates were separated by SDS-PAGE, electro-transferred onto NC membrane, and immunoblotted for the presence of hIL-33-His or hST2-Fc with anti-His HRP or anti-hIgG HRP, respectively.
a) pull down of h-IL-33 by hST2-Fc.
b) pull down of hST2-Fc by h-IL-33-His.
5 μg of hST2-Fc (Prod. No. AG-40A-0059) (or control Fc protein), 2 μg of hIL-33-His (or control His protein), and protein G resin (or anti-His resin) were incubated in 0.5ml RIPA buffer overnight at 4°C. The precipitates were separated by SDS-PAGE, electro-transferred onto NC membrane, and immunoblotted for the presence of hIL-33-His or hST2-Fc with anti-His HRP or anti-hIgG HRP, respectively.
Figure 2: Specific interaction of human ST2 (Prod. No. AG-40A-0059) with recombinant human IL-33.
An indirect competitive ELISA was performed as follows; 1) coat microtiter plate wells with hST2-Fc (10μg/ml); 2) add a varying concentrations of hIL-33 with or without a hIL-33 mAb to the wells followed by washing; 3) add anti-FLAG HRP conjugated (1:2,000) to an enzyme; 4) After adding the TMB solution, incubate at RT in the dark for 10 to 45 minutes. Immediately read the plate at 450 nm.
An indirect competitive ELISA was performed as follows; 1) coat microtiter plate wells with hST2-Fc (10μg/ml); 2) add a varying concentrations of hIL-33 with or without a hIL-33 mAb to the wells followed by washing; 3) add anti-FLAG HRP conjugated (1:2,000) to an enzyme; 4) After adding the TMB solution, incubate at RT in the dark for 10 to 45 minutes. Immediately read the plate at 450 nm.
| Product Details | |
|---|---|
| Synonyms | Interleukin-1 Receptor-like 1; IL-1 Receptor-like 1; IL1RL1; IL-33 Receptor; Interleukin-33 Receptor |
| Product Type | Protein |
| Properties | |
| Source/Host | HEK 293 cells |
| Sequence |
Signal peptide and the sequence of isoform 2 of human ST2 (aa 1-328) are fused at the C-terminus to the Fc portion of human IgG1. |
| Crossreactivity | Human |
| Specificity |
Binds to human IL-33. |
| Biological Activity |
Interacts with human IL-33. |
| MW | ~90kDa (SDS-PAGE) |
| Purity | ≥90% (SDS-PAGE) |
| Endotoxin Content | <0.1EU/μg purified protein (LAL test; Lonza). |
| Concentration | ~0.5mg/ml |
| Formulation | Liquid. 0.2μm-filtered solution in PBS, pH 7.2. |
| Protein Negative Control | |
| Other Product Data |
UniProt link Q01638: ST2 (human) [Precursor] |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice |
After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. For maximum product recovery after thawing, centrifuge the vial before opening the cap. |
| Use/Stability |
Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C. |
| Documents | |
| MSDS |
 Download PDF |
| Product Specification Sheet | |
| Datasheet |
Download PDF |
Description
The ST2 (Interleukin-1 receptor-like 1; Interleukin-33 receptor) gene was originally identified as a gene induced by serum or oncogene expression in fibroblasts. The gene produces a shorter soluble secreted form (ST2) and a longer, transmembrane form (ST2L) by alternative splicing. Soluble ST2 has been shown to downregulate the expression of TLR1 and TLR4. ST2L negatively regulates TLR4 signaling and induces endotoxin tolerance, and enhances Th2 responses. IL-33 is the specific ligand for ST2L.