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Resistin (human) (rec.)

AG-40A-0010Y-C010 10 µg INQ
AG-40A-0010Y-C050 50 µg INQ
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Additional Information

Product Data
Synonyms FIZZ3; Found in Inflammatory Zone 3; ADSF; Adipose Tissue-Specific Secretory Factor
Properties
Source/Host HEK 293 cells
Sequence Human resistin (aa 19-108) is fused at the N-terminus to a FLAG®-tag.
Crossreactivity Human
Biological Activity Inhibits insulin-stimulated glucose uptake in L6 skeletal muscle cells.
MW ~13kDa (SDS-PAGE)
Purity ≥95% (SDS-PAGE)
Endotoxin Content <0.01EU/μg purified protein (LAL test; Lonza).
Concentration After reconstitution:
for 10µg size: 0.1mg/ml
for 50µg size: 1 mg/ml
Reconstitution 10µg size: Reconstitute with 100µl sterile water.
50µg size: Reconstitute with 50µl sterile water.
PBS containing at least 0.1% BSA should be used for further dilutions.
Formulation Lyophilized. Contains PBS.
Other Product Data

UniProt link Q9HD89: Resistin (human) (precursor)

FLAG is a registered trademark of Sigma-Aldrich Co.

Product Type Protein
Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. PBS containing at least 0.1% BSA should be used for further dilutions.
Use/Stability Stable for at least 6 months after receipt when stored at -20°C.
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Product Description

The adipocytokine resistin which belongs to a family of cysteine-rich C-terminal proteins known as resistin-like molecules (RELM; RELM-α/FIZZ 1 and RELM-β/FIZZ 2) of FIZZ (found in inflammatory zone) are thought to be involved in inflammatory processes. Previous studies in mice showed that resistin impairs glucose tolerance and insulin action. In addition, resistin also inhibits adipogenesis in murine 3T3-L1 cells. Therefore resistin has also been proposed as an adipocyte-secreted factor thought to link obesity and T2DM.

Product References

Expression of resistin in the prostate and its stimulatory effect on prostate cancer cell proliferation: H.J. Kim, et al.; BJU Int. 108: 1464 (2010)
Effect of Resistin (human) (rec.) (AG-40A-0010Y) on glucose uptake in L6 cells.  Inhibition of insulin-stimulated glucose uptake in L6 skeletal muscle cells by resistin (as expected: 1μg/ml). Rat myoblasts (L6) were cultured and differentiated into myotubes. Differentiated L6 cells were exposed to resistin for various time and then incubated with or without insulin.
Effect of Resistin (human) (rec.) (AG-40A-0010Y) on glucose uptake in L6 cells. Inhibition of insulin-stimulated glucose uptake in L6 skeletal muscle cells by resistin (as expected: 1μg/ml). Rat myoblasts (L6) were cultured and differentiated into myotubes. Differentiated L6 cells were exposed to resistin for various time and then incubated with or without insulin.
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