AdipoGen Life Sciences

GITRL, Soluble (mouse) (rec.)

CHF 350.00
In stock
AG-40A-0008-C05050 µgCHF 350.00
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Product Details
Synonyms Glucocorticoid-induced TNF Receptor Ligand; AITRL; Activation-inducible TNF-related Ligand; TNFSF18
Product Type Protein
Properties
Source/Host HEK 293 cells
Sequence

The extracellular domain of mouse GITRL (aa 45-173) is fused at the N-terminus to a FLAG®-tag.

Crossreactivity Mouse
Specificity

Binds to mouse GITR.

Biological Activity

Induces GITR-mediated NF-κB activation in HEK 293 cells.

MW ~22kDa (SDS-PAGE)
Purity ≥90% (SDS-PAGE)
Endotoxin Content <0.1EU/μg purified protein (LAL test; Lonza).
Concentration 0.5mg/ml
Formulation Liquid. 0.2μm-filtered solution in 10mM glycine, pH 10.0.
Other Product Data

UniProt link Q7TS55: GITR Ligand (mouse)

FLAG is a registered trademark of Sigma-Aldrich Co.

Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice After opening, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.
For maximum product recovery after thawing, centrifuge the vial before opening the cap.
Use/Stability Stable for at least 6 months after receipt when stored at -20°C.
Working aliquots are stable for up to 3 months when stored at -20°C.
Documents
MSDS Download PDF
Product Specification Sheet
Datasheet Download PDF
Description

GITRL (Glucocorticoid-induced TNF receptor ligand) is expressed on dendritic cells (DC), monocytes, macrophages, B cells, activated T cells, endothelial cells, osteoclasts and various healthy non-lymphoid tissues (e.g. testis). GITRL is constitutively expressed and released as soluble form by solid tumors and various hematopoietic malignancies. GITRL causes differentiation of osteoclasts, activation of macrophages, but also alteration of carcinoma and leukemia cells and influences apoptosis. Binding to GITR is important in regulating T cell proliferation and TCR-mediated apoptosis. GITRL is implicated in development of autoimmune diseases and in the immune response against infectious pathogens and tumors.

Product References

Identification of a ligand for glucocorticoid-induced tumor necrosis factor receptor constitutively expressed in dendritic cells: K.Y. Yu, et al.; BBRC 310, 433 (2003)

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