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AdipoGen Life Sciences
anti-Netrin-1 (human), mAb (rec.) (blocking) (2F5) (preservative-free)
420
CHF
CHF 420.00
In stock
AG-27B-0018PF-C100100 µgCHF 420.00
AG-27B-0018PF-C500500 µgCHF 1'140.00
Figure 1: Schematic antibody structure of the recombinant antibody anti-Netrin-1 (human), mAb (rec.) (blocking) (2F5) (Prod. No. AG-27B-0018PF). The single chain variable human fragment (ScFv) selected by antibody phage display technology and specific to the antigen of interest is fused to a human IgG2 Fc region.
Figure 2: anti-Netrin-1 (human), mAb (rec.) (blocking) (2F5) (preservative free) (Prod. No. AG-27B-0018PF) blocks tumor growth in vivo.
Method: Tumor cells (OCI-Ly3) were implanted in SCID mice by subcutaneous injection of 3x106 cells in 100µl of PBS. When tumors reached 150mm3, mice received intraperitoneal injections of blocking anti-Netrin-1 mAb (2F5) at 20 mg/kg or an equal volume of the antibody control anti-Netrin-1 (human), mAb (rec.) (H4) (preservative free) (Prod. No. AG-27B-0020PF) every two days. Tumor growth rates from the beginning of treatment are shown.
Method: Tumor cells (OCI-Ly3) were implanted in SCID mice by subcutaneous injection of 3x106 cells in 100µl of PBS. When tumors reached 150mm3, mice received intraperitoneal injections of blocking anti-Netrin-1 mAb (2F5) at 20 mg/kg or an equal volume of the antibody control anti-Netrin-1 (human), mAb (rec.) (H4) (preservative free) (Prod. No. AG-27B-0020PF) every two days. Tumor growth rates from the beginning of treatment are shown.
| Product Details | |
|---|---|
| Synonyms | NTN1; NP01-014 |
| Product Type | Recombinant Antibody |
| Properties | |
| Clone | 2F5 |
| Isotype | Human IgG2λ. |
| Source/Host | Produced without the use of animals. Purified from HEK 293 cell culture supernatant. |
| Immunogen/Antigen | Recombinant human Netrin-1. |
| Label/Conjugates | Preservative Free |
| Application |
ELISA |
| Crossreactivity |
Human Mouse |
| Specificity |
Recognizes human and mouse Netrin-1. Does not recognize Netrin-4. Blocks human and mouse Netrin-1 activity on tumor cell lines in vitro and in vivo. Inhibits Netrin-1-enhanced infectivity of Hepatitis C Virus particles. |
| Purity | ≥95% (SDS-PAGE) |
| Purity Detail | Protein A-affinity purified. |
| Concentration | 1mg/ml |
| Formulation | Liquid. In PBS. |
| Isotype Negative Control | |
| Other Product Data |
anti-Netrin-1 (human), mAb (rec.) (blocking) (2F5) is composed of human variable regions (VH and VL) (λ-chain) of immunoglobulin fused to the human lgG2 Fc domain. |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice |
After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. |
| Use/Stability | Stable for at least 1 year after receipt when stored at -20°C. |
| Documents | |
| MSDS |
 Download PDF |
| Product Specification Sheet | |
| Datasheet |
Download PDF |
Description
Netrin-1 controls guidance of CNS commissural axons and peripheral motor axons. Its association with either DCC or some UNC5 receptors will lead to axon attraction or repulsion, respectively. It also serves as a survival factor via its association with its receptors which prevent the initiation of apoptosis. Netrin-1 is also involved in tumorigenesis by regulating apoptosis. Netrin-1 promotes atherosclerosis by retaining macrophages in the artery wall. Netrin-1 also governs induced pluripotent stem cell (iPS) formation and improves reprogramming efficiency of human and mouse somatic cells by limiting apoptosis mediated by Netrin-1 receptors DCC or UNC5b.
Product References
- Netrin-1 regulates somatic cell reprogramming and pluripotency maintenance: D. Ozmandenci, et al.; Nat. Commun. 6, 7398 (2015)
- Epidermal Growth Factor Receptor-Dependent Mutual Amplification between Netrin-1 and the Hepatitis C Virus: M.L. Plissonnier, et al.; PLoS Biol. 14, e1002421 (2016)
- Targeting netrin-1/DCC interaction in diffuse large B-cell and mantle cell lymphomas: T. Broutier, et al.; EMBO Mol. Med. 8, 96 (2016)
- Netrin-1 acts as a non-canonical angiogenic factor produced by human Wharton’s jelly mesenchymal stem cells (WJ-MSC): C.P. Prieto, et al.; Stem Cell Res. Ther. 8, 43 (2017)
- Downregulation of the Netrin-1 Receptor UNC5b Underlies Increased Placental Angiogenesis in Human Gestational Diabetes Mellitus: C.P. Prieto, et al.; Int. J. Mol. Sci. 20, 1408 (2019)
- Netrin1 deficiency activates MST1 via UNC5B receptor, promoting dopaminergic apoptosis in Parkinson’s disease: E.H. Ahn, et al.; PNAS 117, 24503 (2020)
- Netrin-1 and its receptor DCC modulate survival and death of dopamine neurons and Parkinson’s disease features: M. Jasmin, et al.; EMBO J. 40, e105537 (2021)
- Netrin-1 receptor UNC5C cleavage by active δ-secretase enhances neurodegeneration, promoting Alzheimer's disease pathologies: G. Chen, et al.; Sci. Adv. 7, eabe4499 (2021)
- UNC5C Receptor Proteolytic Cleavage by Active AEP Promotes Dopaminergic Neuronal Degeneration in Parkinson’s Disease: G. Chen, et al.; Adv. Sci. 9, e2103396 (2022)
- Netrin-1 Secreted by Human Osteoarthritic Articular Chondrocytes Promotes Angiogenesis in Vitro: J. Akoum, et al.; Cartilage 13, 94 (2022)
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