AdipoGen Life Sciences

anti-IL-33 (mouse), mAb (rec.) (blocking) (Bondy-1-1)

CHF 455.00
In stock
AG-27B-0013-C100100 µgCHF 455.00
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Product Details
Synonyms Interleukin-33; IL-1F11; NF-HEV
Product Type Recombinant Antibody
Properties
Clone Bondy-1-1
Isotype Mouse IgG2b
Source/Host Produced without the use of animals. Purified from HEK 293 cell culture supernatant.
Immunogen/Antigen Mouse recombinant IL-33.
Application

ELISA
Functional: Inhibits the binding of mouse IL-33 to ST2/IL-1RAcP.

Crossreactivity Mouse
Specificity

Recognizes mouse IL-33.

Purity ≥95% (SDS-PAGE)
Purity Detail Protein A-affinity purified.
Endotoxin Content <0.01EU/μg purified protein (LAL test; Lonza).
Concentration 1mg/ml
Formulation Liquid. In PBS containing 10% glycerol and 0.02% sodium azide.
Isotype Negative Control

Fc (mouse) IgG2b Control (rec.)

Other Product Data

anti-IL-33 (mouse), monoclonal antibody (recombinant) (Bondy-1-1) is composed of human variable regions (VH and VL) (λ-chain) of immunoglobulin fused to the mouse lgG2b Fc domain.

Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice After opening, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.
Use/Stability Stable for at least 1 year after receipt when stored at -20°C.
Documents
MSDS Download PDF
Product Specification Sheet
Datasheet Download PDF
Description

Interleukin-33 (IL-33; HF-NEV; IL-1F11), a member of the IL-1 family of cytokines, is expressed by many cell types following pro-inflammatory stimulation and is thought to be released upon cell lysis. IL-33 binds to and signals through ST2 (IL-1R1) and its stimulation recruits MYD88, IRAK, IRAK4 and TRAF6, followed by phosphorylation of ERK1 (MAPK3)/ERK2 (MAPK1), p38 (MAPK14) and JNK. The ability of IL-33 to target numerous immune cell types, like Th2-like cells, mast cells and B1 cells, and to induce cytokine and chemokine production underlines its potential in influencing the outcome of a wide range of diseases, such as arthritis, asthma, atopic allergy & anaphylaxis, cardiovascular disease/atherosclerosis, nervous system diseases and sepsis.

Anti-IL-33, mAb (recombinant) (blocking) (Bondy-1-1) is an antibody developed by antibody phage display technology using a human naive antibody gene library. These libraries consist of scFv (single chain fragment variable) composed of VH (variable domain of the human immunoglobulin heavy chain) and VL (variable domain of the human immunoglobulin light chain) connected by a polypeptide linker. The antibody fragments are displayed on the surface of filamentous bacteriophage (M13). This scFv was selected by affinity selection on antigen in a process termed panning. Multiple rounds of panning are performed to enrich for antigen-specific scFv-phage. Monoclonal antibodies are subsequently identified by screening after each round of selection. The selected monoclonal scFv is cloned into an appropriate vector containing a Fc portion of interest and then produced in mammalian cells to generate an IgG like scFv-Fc fusion protein.

Product References
  1. Regulation of de novo adipocyte differentiation through crosstalk between adipocytes and pre-adipocytes: T.D. Challa, et al.; Diabetes 64, 4075 (2015)
  2. Male-specific IL-33 expression regulates sex-dimorphic EAE susceptibility: A.E. Russi, et al.; PNAS 115, E1520 (2018)
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