Additional Information
| Product Data | |
|---|---|
| Synonyms | Ras-related Protein Rab-6 |
| Properties | |
| Clone | AA2 |
| Isotype | Human IgG2λ |
| Source/Host | Purified from HEK 293 cell culture supernatant. |
| Immunogen/Antigen | Recombinant Rab6AQ72L, a GTP-locked mutant of Rab6A in which Gln72 is replaced by Leu. |
| Application | Immunocytochemistry: (1:1'000) Western Blot: (1:1’000) |
| Crossreactivity | Drosophila, Human, Mouse |
| Specificity | Recognizes human, mouse and Drosophila GTP-bound Rab6a and Rab6b and mutant Rab6Q72L. Does not detect Rab6•GDP. |
| Purity | ≥95% (SDS-PAGE) |
| Purity Detail | Protein A-affinity purified. |
| Concentration | 1mg/ml |
| Formulation | Liquid. In PBS containing 10% glycerol and 0.02% sodium azide. |
| Other Product Data | anti-Rab6-GTP monoclonal antibody (recombinant) (AA2) is composed of human variable regions (VH and VL) (λ-chain) of immunoglobulin fused to the human lgG2 Fc domain.
anti-Rab6-GTP monoclonal antibody (recombinant) (AA2) is an antibody developed by antibody phage display technology using a human naive antibody gene library. These libraries consist of scFv (single chain fragment variable) composed of VH (variable domain of the human immunoglobulin heavy chain) and VL (variable domain of the human immunoglobulin light chain) connected by a polypeptide linker. The antibody fragments are displayed on the surface of filamentous bacteriophage (M13). This scFv was selected by affinity selection on antigen in a process termed panning. Multiple rounds of panning are performed to enrich for antigen-specific scFv-phage. Monoclonal antibodies are subsequently identified by screening after each round of selection. The selected monoclonal scFv is cloned into an appropriate vector containing a Fc portion of interest and then produced in mammalian cells to generate an IgG like scFv-Fc fusion protein. |
| Product Type | Recombinant Antibody |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice | After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. |
| Use/Stability | Stable for at least 1 month after receipt when stored at +4°C. Stable for at least 1 year after receipt when stored at -20°C. |
| Documents | |
| Protocols |
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| MSDS |
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Product Description
Rab6 is involved in protein transport. It is a regulator of membrane traffic from the Golgi apparatus towards the endoplasmic reticulum (ER).
Product References
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Rab6-GTP is detected by immunocytochemistry using anti-Rab6-GTP, mAb (AA2) (Prod. No. AG-27B-0004).
Method: HeLa cells are grown in standard culture conditions, fixed with paraformaldehyde (3%), permeablized in PBS+ BSA 0.2 % + Saponin 0.05 % and incubated with anti-Rab6-GTP, mAb (AA2) (1μg /ml) in PBS-BSA-Saponin. After incubation for 30 min at RT and several washes in PBS, cells are treated with a goat anti-human (Cy3) antibody in PBS-BSA-Saponin for 30 min at RT, washed and mounted in Moewiol. Nuclei are stained with DAPI.
Picture courtesy of Dr Moutel, Dr Franck Perez Lab, Curie Institute, Paris.
Method: HeLa cells are grown in standard culture conditions, fixed with paraformaldehyde (3%), permeablized in PBS+ BSA 0.2 % + Saponin 0.05 % and incubated with anti-Rab6-GTP, mAb (AA2) (1μg /ml) in PBS-BSA-Saponin. After incubation for 30 min at RT and several washes in PBS, cells are treated with a goat anti-human (Cy3) antibody in PBS-BSA-Saponin for 30 min at RT, washed and mounted in Moewiol. Nuclei are stained with DAPI.
Picture courtesy of Dr Moutel, Dr Franck Perez Lab, Curie Institute, Paris.
Structure of the recombinant antibody anti-Rab6 (h)-GTP, mAb (rec.) (AA2) (Prod. No. AG-27B-0004). The single chain variable human fragment (ScFv) selected by antibody phage display technology and specific to the antigen of interest is fused to a human IgG2 Fc region.
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