Additional Information
| Product Data | |
|---|---|
| Synonyms | Golgin Subfamily B Member 1; 372 kDa Golgi Complex-associated Protein; GCP372; Macrogolgin |
| Properties | |
| Clone | TA10 |
| Isotype | Human IgG2λ |
| Source/Host | Purified from HEK 293 cell culture supernatant. |
| Immunogen/Antigen | Human recombinant giantin. |
| Label/Conjugates | ATTO 488 |
| Application | Immunocytochemistry: (1:1'000) |
| Crossreactivity | Human, Mouse |
| Specificity | Recognizes human and mouse giantin. |
| Purity | ≥95% (SDS-PAGE) |
| Purity Detail | Protein A-affinity purified. |
| Concentration | 1mg/ml |
| Formulation | Liquid. In PBS containing 0.02% sodium azide. |
| Other Product Data | anti-Giantin, monoclonal antibody (recombinant) (TA10) is composed of human variable regions (VH and VL) (λ-chain) of immunoglobulin fused to the human lgG2 Fc domain.
anti-Giantin, monoclonal antibody (recombinant) (TA10) is an antibody developed by antibody phage display technology using a human naive antibody gene library. These libraries consist of scFv (single chain fragment variable) composed of VH (variable domain of the human immunoglobulin heavy chain) and VL (variable domain of the human immunoglobulin light chain) connected by a polypeptide linker. The antibody fragments are displayed on the surface of filamentous bacteriophage (M13). This scFv was selected by affinity selection on antigen in a process termed panning. Multiple rounds of panning are performed to enrich for antigen-specific scFv-phage. Monoclonal antibodies are subsequently identified by screening after each round of selection. The selected monoclonal scFv is cloned into an appropriate vector containing a Fc portion of interest and then produced in mammalian cells to generate an IgG like scFv-Fc fusion protein. |
| Product Type | Recombinant Antibody |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Short Term Storage | +4°C |
| Long Term Storage | +4°C |
| Use/Stability | Keep conjugated formats at +4°C. Stable for at least 1 year after receipt when stored at +4°C. |
| Documents | |
| Protocols |
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| MSDS |
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Product Description
Giantin, the largest Golgi membrane protein, is C-terminally anchored to cis- and medial-Golgi membranes and forms a long ~250nm coiled-coil rod that directly interacts with microtubules in a cell-cycle regulated way. It is an excellent marker for mammalian golgi complex. It is also an antigen in chronic rheumatoid arthritis and in the autoimmune disease Sjoegren syndrome.
Product References
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Human giantin is detected by immunocytochemistry using anti-Giantin, mAb (rec.) (TA10) (ATTO 488) (Prod. No. AG-27B-0003TD).
Method: HeLa cells are grown in standard culture conditions, fixed with methanol, and incubated with using anti-Giantin, mAb (rec.) (TA10) (ATTO 488) (Prod. No. AG-27B-0003TD) (1μg /ml in PBS-BSA). After incubation for 30 min at RT and several washes in PBS, cells are mounted in Moewiol. Nuclei are stained with DAPI.
Picture courtesy of Dr. Moutel, Dr. Franck Perez Lab, Curie Institute, Paris.
Method: HeLa cells are grown in standard culture conditions, fixed with methanol, and incubated with using anti-Giantin, mAb (rec.) (TA10) (ATTO 488) (Prod. No. AG-27B-0003TD) (1μg /ml in PBS-BSA). After incubation for 30 min at RT and several washes in PBS, cells are mounted in Moewiol. Nuclei are stained with DAPI.
Picture courtesy of Dr. Moutel, Dr. Franck Perez Lab, Curie Institute, Paris.
Structure of the recombinant antibody anti-Giantin (h), mAb (rec.)(TA10) (Prod. No. AG-27B-0003). The single chain variable human fragment (ScFv) selected by antibody phage display technology and specific to the antigen of interest is fused to a human IgG2 Fc region.
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