Additional Information
| Product Data | |
|---|---|
| Synonyms | Poly(ADP-ribose) Polymerase 1; ADP-ribosyltransferase Diphtheria Toxin-like 1; NAD(+) ADP-ribosyltransferase 1 |
| Properties | |
| Source/Host | Rabbit |
| Immunogen/Antigen | Recombinant human PARP-1 [ARTD1] (aa 215-525). |
| Application | Immunocytochemistry: 1:500-1:1'000 Immunohistochemistry: 1:500-1:1'000 Immunoprecipitation: 2μl per 100μg nuclear extract Western Blot: 1:2'000-1:5'000 Optimal conditions must be determined individually for each application. |
| Crossreactivity | Human, Monkey, Mouse |
| Specificity | Recognizes human PARP-1. Cross-reacts weakly with mouse and monkey PARP-1. |
| Formulation | Liquid. Contains 0.1% sodium azide. |
| Other Product Data | UniProt ID P09874: PARP-1 (human) |
| Product Type | Polyclonal Antibody |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice | After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. |
| Use/Stability | Stable for at least 1 year after receipt when stored at -20°C. |
| Documents | |
| MSDS | 
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Product Description
PARP-1 (ARTD1) is involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. PARP-1 positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150. It forms a complex with EEF1A1 and TXK that acts as a T-helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-γ to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production.
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