|Synonyms||Poly(ADP-ribose) Polymerase 1; ADP-ribosyltransferase Diphtheria Toxin-like 1; NAD(+) ADP-ribosyltransferase 1|
|Immunogen/Antigen||Recombinant human PARP-1 [ARTD1] (aa 215-525).|
Immunoprecipitation: 2μl per 100μg nuclear extract
Western Blot: 1:2'000-1:5'000
Optimal conditions must be determined individually for each application.
|Crossreactivity||Human, Monkey, Mouse|
|Specificity||Recognizes human PARP-1. Cross-reacts weakly with mouse and monkey PARP-1.|
|Formulation||Liquid. Contains 0.1% sodium azide.|
|Other Product Data||UniProt ID P09874: PARP-1 (human)|
|Product Type||Polyclonal Antibody|
|Shipping and Handling|
|Short Term Storage||+4°C|
|Long Term Storage||-20°C|
|Handling Advice||After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.|
|Use/Stability||Stable for at least 1 year after receipt when stored at -20°C.|
PARP-1 (ARTD1) is involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. PARP-1 positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150. It forms a complex with EEF1A1 and TXK that acts as a T-helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-γ to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production.
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