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AdipoGen Life Sciences
anti-Fis1, pAb
420
CHF
CHF 420.00
In stock
AG-25B-0007V-C100100 µgCHF 420.00
Figure 1: Immunocytochemical staining and detection of endogenous Fis1 (red) (right) and endogenous mitochondrial Hsp70 (green) (left) in methanol fixed HeLa cells using anti-Fis1, pAb (Prod. No. AG-20B-0007V).
Picture courtesy of P. Parone, University of Geneva.
Picture courtesy of P. Parone, University of Geneva.
Figure 2: Localisation of endogenous human Fis1 in HeLa cells. Method: Double staining with mtHSP70, mAb (JG1) [left] and using anti-Fis1, pAb (Prod. No. AG-20B-0007V) [right] on methanol fixed cells reveals mitochondrial localization.
Figure 3: Detection of endogenous Fis1 by Western blot (1:1'000) using anti-Fis1, pAb (Prod. No. AG-20B-0007V).
Method: 10μg mitochondrial proteins from mouse brain (A) and kidney (B); 10μg cytosolic proteins from mouse liver (C).
Method: 10μg mitochondrial proteins from mouse brain (A) and kidney (B); 10μg cytosolic proteins from mouse liver (C).
| Product Details | |
|---|---|
| Synonyms | Mitochondrial Fission 1 Protein; Tetratricopeptide Repeat Protein 11; TTC 11 |
| Product Type | Polyclonal Antibody |
| Properties | |
| Source/Host | Rabbit |
| Immunogen/Antigen | Recombinant human Fis1. |
| Application |
Immunocytochemistry: (methanol fixation; 1:200) |
| Crossreactivity |
Human Mouse Rat |
| Specificity |
Recognizes human, mouse and rat Fis1. |
| Purity Detail | Protein A-affinity purified. |
| Concentration | 1mg/ml |
| Formulation | Liquid. In PBS containing 10% glycerol and 0.02% sodium azide. |
| Isotype Negative Control | |
| Other Product Data |
This purified antiserum is replacing the product AG-25B-0007 Fis-1 pAb (neat antiserum) with same specifications. |
| Declaration | Manufactured by AdipoGen. Formerly sold as ALX-210-907 by ALEXIS Biochemicals. |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice |
After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. |
| Use/Stability | Stable for at least 1 year after receipt when stored at -20°C. |
| Documents | |
| MSDS |
 Download PDF |
| Product Specification Sheet | |
| Datasheet |
Download PDF |
Description
Fis1 promotes the fragmentation of the mitochondrial network and its perinuclear clustering. It can induce cytochrome c release from mitochondria to the cytosol, ultimately leading to apoptosis. It also mediates peroxisomal fission.
Product References
- hFis1, a novel component of the mammalian mitochondrial fission machinery: D.I. James, et al.; J. Biol. Chem. 278, 36373 (2003)
- A role for Fis1 in both mitochondrial and peroxisomal fission in mammalian cells: A. Koch, et al.; Mol. Biol. Cell 16, 5077 (2005)
- The Novel Tail-anchored Membrane Protein Mff Controls Mitochondrial and Peroxisomal Fission in Mammalian Cells.: S. Gandre-Babbe & AM. van der Bliek; Mol. Biol. Cell 6, 2402 (2008)
- Mitochondrial dysfunction in hereditary spastic paraparesis with mutations in DDHD1/SPG28: A. Mignarri, et al.; J. Neurol. Sci. 362, 287 (2016)