AdipoGen Life Sciences

anti-Ribosomal Protein S3 (human), pAb

CHF 390.00

In stock

AG-25A-0077-C100100 µgCHF 390.00

Figure 1: Immunohistochemical staining of RPS3 using using anti-RPS3 (human), pAb (Prod. No. AG-25A-0077) in human colon tissue (5µg/ml).
This antibody has been tested in immunohistochemistry, analyzed by an anatomic pathologist and validated for use in IHC applications against formalin-fixed, paraffin-embedded human tissues. The image shows the localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.

Figure 2: Immunocytochemical staining of RPS3 using using anti-RPS3 (human), pAb (Prod. No. AG-25A-0077) in HT1080 cells (1:100 dilution). Immunofluorescence experiments were performed to determine the intracellular localization of rpS3 in the cytosol and membrane ruffle. (A) x40, (B) x100 objective, respectively.

Figure 3: Western blot and Immunoprecipitation analysis using anti-RPS3 (human), pAb (Prod. No. AG-25A-0077).
Western Blot (1:1'000 dilution):
1: Vector alone-transfected HEK 293 cell lysate (V).
2: RPS3 (FLAG^®-tagged)-transfected HEK 293 cell lysate (FS3).

Immunoprecipitation (1:500 dilution):
3. Anti-mouse IgG.
4. Anti-FLAG.
5. Anti-mouse IgG.
6. Anti-FLAG.
7. Anti-rabbit IgG.
8. Anti-RPS3 pAb.
9. Anti-rabbit IgG.
10. Anti-rabbit RPS3 pAb.

Specifications / Handling

More Information
Product Details
Synonyms	40S Ribosomal Protein S3; IMR-90 Ribosomal Protein S3; RPS3
Product Type	Polyclonal Antibody
Properties
Source/Host	Rabbit
Immunogen/Antigen	Recombinant human ribosomal protein S3.
Application	ELISA: (direct and indirect: 1:2’000-1:5’000) Immunocytochemistry: (1:100) Immunohistochemistry: (5μg/ml) Immunoprecipitation: (1:500-1:5’000) Western Blot: (1:500-1:5’000 using ECL. Suggested blocking and dilution buffer is PBST containing 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at room temperature). Optimal conditions must be determined individually for each application.
Crossreactivity	Human
Specificity	Recognizes human ribosomal protein S3. Detects a band of ~32kDa by Western blot.
Purity Detail	Protein A-affinity purified.
Concentration	1mg/ml
Formulation	Liquid. 0.2μm-filtered solution in PBS, pH 7.4. Contains no preservatives.
Isotype Negative Control	Rabbit IgG
Shipping and Handling
Shipping	BLUE ICE
Short Term Storage	+4°C
Long Term Storage	-20°C
Handling Advice	After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
Use/Stability	Stable for at least 6 months after receipt when stored at -20°C.
Documents
MSDS	Download PDF
Product Specification Sheet
Datasheet	Download PDF

Product-specific References

Description

RpS3 is a component of the 40S ribosomal subunit and is an an essential but previously unkown subunit of NF-κB involved in the regulation of key genes in rapid cellular activation responses. RpS3 interacts with nm23-H1. The expression of rpS3 reduces the secretion of MMP-9 and the invasive metastatic potential in HT1080 cells. The phosphorylated ERK is reduced by the expression of rpS3.

Product References

Interaction of Hsp90 with ribosomal proteins protects from ubiquitination and proteasome-dependent degradation: T.S. Kim, et al.; Mol. Biol. Cell 17, 824 (2006)
Reduction of invasion in human fibrosarcoma cells by ribosomal protein S3 in conjunction with Nm23-H1 and ERK: S.H. Kim & J. Kim; Biochim. Biophys. Acta 1763, 823 (2006)
Arginine methylation of ribosomal protein S3 affects ribosome assembly: H.S. Shin, et al.; BBRC 385, 273 (2009)

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