AdipoGen Life Sciences

anti-ST2 (human), pAb

CHF 390.00
In stock
AG-25A-0058-C100100 µgCHF 390.00
More Information
Product Details
Synonyms Interleukin-1 Receptor-like 1; IL-1 Receptor-like 1; IL1RL1; Interleukin-33 Receptor; IL-33-Receptor
Product Type Polyclonal Antibody
Properties
Source/Host Rabbit
Immunogen/Antigen Recombinant human soluble ST2.
Application

ELISA: (direct and indirect: 1:2000-1:5000)
Flow Cytometry: 1/100 dilution
Western Blot: (1:2000-1:5000 using ECL; suggested blocking and dilution buffer is PBST containing 0.05% Tween 20 and 5% skim milk; suggested incubation time is 1 hour at room temperature).
Optimal conditions must be determined individually for each application.

Crossreactivity Human
Specificity

Recognizes soluble human ST2. Detects a band of ~50kDa by Western blot and the endogenous ST2 by Flow Cytometry.

Purity Detail Protein A-affinity purified.
Concentration 1mg/ml
Formulation Liquid. In PBS containing 0.02% sodium azide.
Isotype Negative Control

Rabbit IgG

Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice After opening, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.
Use/Stability Stable for at least 6 months after receipt when stored at -20°C.
Documents
MSDS Download PDF
Product Specification Sheet
Datasheet Download PDF
Description

ST2 is a member of the Toll/IL-1 receptor family. Two forms of the protein exist, a soluble form known as ST2 and a membrane anchored form known as ST2L. The membrane form is expressed by Th2 cells and bone marrow derived mast cells, whereas the soluble form is expressed by serum-stimulated fibroblasts. It binds IL-33 and is structurally similar to IL-1R1. Type 2 innate lymphoid cells (ILC2), originally referred to as natural helper (NH) cells, are a novel target of IL-33. Lung ILC2s participate in the induction of airway inflammation in influenza virus-infected mice and papain-administrated mice. Soluble ST2 plays a role in protecting ILC2 from IL-33 stimulation and thereby maintaining them in a naïve state and might be important for the regulation of several disease. Blocking with anti-ST2 antibodies has been shown to alleviate experimental arthritis and airway inflammation. The IL-33-ST2 axis is is involved across a range of diseases including asthma, allergies, obesity and cardiac disease.

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