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anti-IDO (mouse), pAb

AG-25A-0032-C100 100 µg INQ
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Additional Information

Product Data
Synonyms Indoleamine 2,3-dioxygenase; INDO
Properties
Source/Host Rabbit
Immunogen/Antigen Recombinant mouse IDO.
Application ELISA: (direct and indirect: 1:2’000-1:10’000)
Immunocytochemistry: (1:500-1:2’000)
Immunohistochemistry: (paraffin sections (1:200-1:500))
Western Blot: (1:2’000-1:5’000 using ECL; suggested blocking and dilution buffer is PBST containing 0.05% Tween 20 and 5% skim milk; suggested incubation time is 1 hour at room temperature).
Optimal conditions must be determined individually for each application.
Crossreactivity Mouse
Specificity Recognizes mouse IDO. Detects a band of ~45kDa by Western blot.
Purity Detail Protein A-affinity purified.
Concentration 1mg/ml
Formulation Liquid. 0.2μm-filtered solution in PBS, pH 7.4. Contains no preservatives.
Product Type Polyclonal Antibody
Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
Documents
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Product Description

IDO catalyzes the first and rate-limiting step in the main pathway of human tryptophan catabolism, the kynurenine pathway. Proinflammatory mediators, such as endotoxin and IFN-γ induce the expression of IDO in several tissues. IDO-dependent suppression of T-cell responses might function as natural immunoregulatory mechanism. Physiological IDO activity has been implicated in T-cell tolerance to tumors, dysfunctional selftolerance in non-obese diabetic (NOD) mice, and as a protective negative regulator in autoimmune disorders.

Product References

  1. IFN-gamma-induced IDO and WRS expression in microglia is differentially regulated by IL-4: M.C. Yadav, et al.; Glia 55, 1385 (2007)
  2. Spontaneous renal allograft acceptance associated with "regulatory" dendritic cells and IDO: C.H. Cook, et al.; J. Immunol. 180, 3103 (2008)
  3. Blockade of Indoleamine 2,3-Dioxygenase Protects Mice against Lipopolysaccharide-Induced Endotoxin Shock: I.D. Jung, et al.; J. Immunol. 182, 3146 (2009)
  4. A novel cancer therapy by skin delivery of indoleamine 2,3-dioxygenase siRNA: M.C. Yen, et al.; Clin. Cancer Res. 15, 641 (2009)
  5. Curcumin suppresses the induction of indoleamine 2,3-dioxygenase by blocking the Janus-activated kinase-protein kinase Cdelta-STAT1 signaling pathway in interferon-gamma-stimulated murine dendritic cells: Y.I. Jeong, et al.; J. Biol. Chem. 284, 3700 (2009)
  6. A combination of the metabolic enzyme inhibitor APO866 and the immune adjuvant L-1-methyl tryptophan induces additive antitumor activity: H.J. Yang, et al.; Exp. Biol. Med. (Maywood) 235, 869 (2010)
Immunohistochemical staining of mouse gut using anti-IDO (mouse), pAb (Prod. No. AG-25A-0032). Colorization by ABS Reagent (Vectastain) and counterstaining with hematoxylin.
Immunohistochemical staining of mouse gut using anti-IDO (mouse), pAb (Prod. No. AG-25A-0032). Colorization by ABS Reagent (Vectastain) and counterstaining with hematoxylin.
Western blot analysis of mouse IDO using anti-IDO (mouse), pAb (Prod. No. AG-25A-0032) at 1:2,000 dilution.<br /> 1. Recombinant mouse IDO (His-tagged).<br /> 2. Con-A treated mouse peripheral blood lymphocyte lysate.
Western blot analysis of mouse IDO using anti-IDO (mouse), pAb (Prod. No. AG-25A-0032) at 1:2,000 dilution.
1. Recombinant mouse IDO (His-tagged).
2. Con-A treated mouse peripheral blood lymphocyte lysate.
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