AdipoGen Life Sciences

anti-Adiponectin (human), pAb

CHF 390.00

In stock

AG-25A-0003-C100100 µgCHF 390.00

Figure 1: Western blot analysis in human plasma using anti-Adiponectin (human), pAb (Prod. No. AG-25A-0003) at 0.2μg/ml concentration.

Specifications / Handling

More Information
Product Details
Synonyms	ACRP30; AdipoQ; apM1; GBP28; Adipocyte Complement Related Protein of 30kDa
Product Type	Polyclonal Antibody
Properties
Source/Host	Rabbit
Immunogen/Antigen	Recombinant human adiponectin.
Application	ELISA: (direct and indirect; 1:5'000-1:10'000) Western Blot: (1:5'000-1:10'000 using ECL; suggested blocking and dilution buffer is TBST with 0.05% Tween 20 with 5% skim milk; incubate 1 hour at room temperature). Optimal conditions must be determined individually for each application.
Crossreactivity	Human
Specificity	Recognizes human adiponectin. Detects a band of ~30kDa by Western blot.
Purity Detail	Protein A-affinity purified.
Concentration	1mg/ml
Formulation	Liquid. 0.2μm-filtered solution in PBS, pH 7.4. Contains no preservatives.
Isotype Negative Control	Rabbit IgG
Shipping and Handling
Shipping	BLUE ICE
Short Term Storage	+4°C
Long Term Storage	-20°C
Handling Advice	After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
Use/Stability	Stable for at least 6 months after receipt when stored at -20°C.
Documents
MSDS	Download PDF
Product Specification Sheet
Datasheet	Download PDF

Product-specific References

Description

ACRP30 was identified as a novel adipocyte-specific synthesized and secreted protein with structural resemblance to complement factor C1q. Like adipsin, ACRP30 secretion is induced ~10-fold during adipocyte differentiation. Plasma levels are reduced in obese humans, and low levels are associated with insulin-resistance. Treatment of db/db mice with TZD increased ACRP30 levels.

Product References

Plasma resistin concentrations measured by enzyme-linked immunosorbent assay using a newly developed monoclonal antibody are elevated in individuals with type 2 diabetes mellitus: B.S. Youn, et al.; J. Clin. Endocrinol. Metab. 89, 150 (2004)

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