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AdipoGen Life Sciences
anti-Hairy and Enhancer of Split 1 (HES1), mAb (7H11)
380
CHF
CHF 380.00
In stock
AG-20T-0400-C100100 µgCHF 380.00
Figure 1: Western blot analysis using anti-HES1, mAb (7H11) (Prod. No. AG-20T-0400) in cell lysates. Lane1: HeLa cell lysate; Lane 2: HT29 cell lysate; Lane 3: BeWo cell lysate.
Figure 2: Immunoprecipitation of Hes1 using anti-HES1, mAb (7H11) (Prod. No. AG-20T-0400).
Lane1: BeWo cell lysate;
Lane 2: Precipitated from BeWo cell lysate 400µg, using 7H11 at 2µg;
Lane 3: Precipitated from BeWo cell lysate 400µg, using 7H11 at 5µg;
Lane 4: Precipitated from PBS, using 7H11 at 5µg.
Lane1: BeWo cell lysate;
Lane 2: Precipitated from BeWo cell lysate 400µg, using 7H11 at 2µg;
Lane 3: Precipitated from BeWo cell lysate 400µg, using 7H11 at 5µg;
Lane 4: Precipitated from PBS, using 7H11 at 5µg.
| Product Details | |
|---|---|
| Synonyms | hHL; HES1; BHLHB39; bHLHb39; Hairy Homolog; Hairy-like Protein; Transcription Factor HES-1; Hairy and Enhancer of Split 1; Class B Basic Helix-Loop-Helix Protein 39 |
| Product Type | Monoclonal Antibody |
| Properties | |
| Clone | 7H11 |
| Isotype | Mouse IgG2bκ |
| Immunogen/Antigen | Recombinant human Hes1 protein fused to a His-tag. |
| Application |
ELISA: |
| Crossreactivity |
Human Mouse Rat |
| Specificity |
Recognizes human, mouse and rat HES1. |
| Purity | ≥95% (SDS-PAGE) |
| Purity Detail | Ammonium sulfate precipitation. |
| Concentration | 1mg/ml |
| Formulation | Liquid. HEPES with 0.15M NaCl, 0.01% BSA, 0.03% sodium azide, and 50% glycerol. |
| Isotype Negative Control | |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice | Avoid freeze/thaw cycles. |
| Use/Stability | Stable for at least 1 year after receipt when stored at -20°C. |
| Documents | |
| MSDS |
 Download PDF |
| Product Specification Sheet | |
| Datasheet |
Download PDF |
Description
The Notch signaling pathway is important for cell-cell communication, which involve gene regulation mechanisms that control multiple cell differentiation processes during embryonic and adult life. It critically influences cell proliferation, differentiation, and apoptosis in metazoans. The hairy and enhancer of split (HES) family is a basic helix-loop-helix (bHLH) type trancriptional repressor and acts as Notch effectors by negatively regulating expression of downstream target genes such as tissue-specific transcription factors. HES-1 is an upstream negative regulator of REST expression. Silencing of the transcriptional repressor REST is required for terminal differentiation of neuronal and β-cells. Recent integrative genomic analyses on HES/HEY family suggest that HES1 and HES3 are target genes of the embryonic stem cell-specific network of transcription factors, and that HES1, HES5, HEY1, HEY2 and HEYL are target genes of Notch signaling pathway.
Product References
- Mechanoregulation of h2-calponin gene expression and the role of notch signaling: W. Jiang, et al.; J. Biol. Chem. 289, 1617 (2014)
- CRMP5 controls glioblastoma cell proliferation and survival through Notch-dependent signaling: A. Moutal, et al.; Cancer Res. 75, 3519 (2015)
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