AdipoGen Life Sciences

anti-Caspase-8 (mouse), mAb (1G12)

CHF 350.00
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AG-20T-0137-C100100 µgCHF 350.00
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Product Details
Synonyms Apoptotic Cysteine Protease; Apoptotic Protease Mch-5; CAP4; FADD-homologous ICE/ced-3-like Protease
Product Type Monoclonal Antibody
Properties
Clone 1G12
Isotype Rat IgG1κ
Immunogen/Antigen Recombinant mouse caspase-8 (p20 subunit).
Application

ELISA: (recombinant mouse caspase-8)
Flow Cytometry: (overexpressed in cell lines)
Immunocytochemistry: (see literature reference 2)
Western Blot: (excellent)
Optimal conditions must be determined individually for each application.

Crossreactivity Mouse
Specificity

Recognizes the p20 subunit of mouse caspase-8. Detects bands of ~55kDa (full-length caspase-8) and ~18kDa (apoptosis-induced cleavage fragment) by Western blot. Does not cross-react with human caspase-8.

Purity ≥95% (SDS-PAGE)
Purity Detail Protein G-affinity purified (sepharose column).
Endotoxin Content <0.1EU/μg purified protein (LAL test; Lonza).
Concentration 1mg/ml
Formulation Liquid. In PBS containing 0.02% sodium azide.
Isotype Negative Control

Rat IgG1 Isotype Control

Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice After opening, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.
Use/Stability Stable for at least 1 year after receipt when stored at -20°C.
Documents
MSDS Download PDF
Product Specification Sheet
Datasheet Download PDF
Description

Procaspase-8 belongs to the family of caspases. Binding of FasL to Fas leads to formation of a receptor complex at the cellular membrane, which was named DISC. The DISC consists of oligomerized receptors, the DD-containing adaptor molecule FADD, procaspase-8, procaspase-10 and c-FLIP. The DISC structure provides a platform for the oligomerization of procaspase-8 that allows two procaspase-8 homodimers to be in the close proximity leading to the initial activation of procaspase-8. At the first cleavage step, the N-terminal p43/p41 and the C-terminal p30 cleavage products are generated. Importantly, these cleavage products already possess catalytic activity. At the second cleavage step, p43/p41 and p30 are processed to p10 and p20, respectively, which leads to the generation of the active caspase-8 heterotetramer (p20/p10)2.

Product References
  1. Apaf-1 and caspase-9 do not act as tumor suppressors in myc-induced lymphomagenesis or mouse embryo fibroblast transformation: C.L. Scott, et al.; J. Cell Biol. 164, 89 (2004)
  2. Modifications and intracellular trafficking of FADD/MORT1 and caspase-8 after stimulation of T lymphocytes: L.A. O'Reilly, et al.; Cell Death Differ. 11, 724 (2004)
  3. Caspase-8 serves both apoptotic and nonapoptotic roles: T.B.Kang, et al.; J. Immunol. 73, 2976 (2004)
  4. Role of membrane sphingomyelin and ceramide in platform formation for Fas-mediated apoptosis: M. Miyaji, et al.; J. Exp. Med. 202, 249 (2005)
  5. Fatal Hepatitis Mediated by Tumor Necrosis Factor TNFα Requires Caspase-8 and Involves the BH3-Only Proteins Bid and Bim: T. Kaufmann, et al.; Immunity 30, 56 (2009)
  6. Catalytic activity of the caspase-8-FLIPL complex inhibits RIPK3-dependent necrosis: A. Oberst, et al.; Nature 471, 363 (2011)
  7. FLIP (Flice-like inhibitory protein) suppresses cytoplasmic double-stranded-RNA-induced apoptosis and NF-B and IRF3-mediated signaling: P. Handa, et al.; Cell Commun. Signal. 9, 16 (2011)
  8. XIAP restricts TNF- and RIP3-dependent cell death and inflammasome activation: M. Yabal, et al.; Cell Rep. 7, 1796 (2014)
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