AdipoGen Life Sciences

anti-Caspase-2, mAb (11B4)

CHF 450.00
In stock
AG-20T-0136-C100100 µgCHF 450.00
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Product Details
Synonyms CASP-2; Protease ICH-1; Neural Precursor Cell Expressed Developmentally Down-regulated Protein 2; NEDD-2
Product Type Monoclonal Antibody
Properties
Clone 11B4
Isotype Rat IgG2aκ
Immunogen/Antigen Recombinant human caspase-2 (p19 subunit).
Application

Immunoprecipitation: (1-5μg/ml)
Western Blot: (1-2μg/ml)
Optimal conditions must be determined individually for each application.

Crossreactivity Dog
Human
Monkey
Mouse
Rat
Specificity

Recognizes an epitope in the p19 subunit of human, mouse, rat, monkey and dog caspase-2. Detects bands of ~51kDa (procaspase-2), ~32kDa and ~18kDa (cleavage products) by Western blot.

Purity ≥95% (SDS-PAGE)
Purity Detail Protein G-affinity purified (sepharose column).
Endotoxin Content <0.1EU/μg purified protein (LAL test; Lonza).
Concentration 1mg/ml
Formulation Liquid. In PBS containing 0.02% sodium azide.
Isotype Negative Control

Rat IgG2a Isotype Control

Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice After opening, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.
Use/Stability Stable for at least 1 year after receipt when stored at -20°C.
Documents
MSDS Download PDF
Product Specification Sheet
Datasheet Download PDF
Description

Caspase-2 is a Class I caspase with a long prodomain necessary for nuclear localization. Upon activation of the apoptotic pathway, the procaspase is cleaved into smaller fragments. Caspase-2 is the nuclear apoptotic respondent to cellular genotoxic stress or mitotic catastrophe and is involved in the activation cascade of caspases responsible for apoptosis execution. Activation occurs upon recruitment to a complex containing a p53-induced death domain protein, PIDD. This suggests caspase-2 can be a nuclear initiator caspase with a requirement for caspase-9 and caspase-3 activation in downstream apoptotic events. In apoptotic pathways resulting from UV-induced DNA damage, processing of caspase-2 occurs downstream of mitochondrial dysfunction and of caspase-9 and caspase-3 activation, extending a possible role for caspase-2 as a parallel effector caspase.

Product References
  1. Caspase-2 is not required for thymocyte or neuronal apoptosis even though cleavage of caspase-2 is dependent on both Apaf-1 and caspase-9: L.A. O'Reilly, et al.; Cell Death Differ. 9, 832 (2002)
  2. A novel Apaf-1–independent putative caspase-2 activation complex: S.H. Read, et al.; J. Cell Biol. 159, 739 (2002)
  3. A Novel Caspase-2 Complex Containing TRAF2 and RIP1: M. Lamkanfi, et al.; J. Biol. Chem. 280, 6923 (2005)
  4. Death domain assembly mechanism revealed by crystal structure of the oligomeric PIDDosome core complex: H.H. Park, et al.; Cell 128, 533 (2007)
  5. Caspase-2 activation in the absence of PIDDosome formation: C. Manzl, et al.; J. Cell Biol. 185, 291 (2009)
  6. LBH589, a deacetylase inhibitor, induces apoptosis in adult T-cell leukemia/lymphoma cells via activation of a novel RAIDD-caspase-2 pathway: H. Hasegawa, et al.; Leukemia 25, 575 (2011)
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