AdipoGen Life Sciences

anti-Apaf-1 (mouse/rat), mAb (13F11)

CHF 390.00
In stock
AG-20T-0133-C100100 µgCHF 390.00
More Information
Product Details
Synonyms Apoptosis Protease-activating Factor 1
Product Type Monoclonal Antibody
Properties
Clone 13F11
Isotype Rat IgG2aκ
Immunogen/Antigen Recombinant mouse Apaf-1 (aa 1-97) containing the N-terminal CARD domain.
Application

ELISA
Immunocytochemistry
Immunoprecipitation: (1:1000-1:2000)
Western Blot: (1:1000-1:2000)
Optimal conditions must be determined individually for each application.

Crossreactivity Mouse
Rat
Specificity

Recognizes the CARD domain of mouse and rat Apaf-1. Does not cross-react with human Apaf-1.

Purity ≥95% (SDS-PAGE)
Purity Detail Protein G-affinity purified (sepharose column).
Endotoxin Content <0.1EU/μg purified protein (LAL test; Lonza).
Concentration 1mg/ml
Formulation Liquid. In PBS containing 0.02% sodium azide.
Isotype Negative Control

Rat IgG2a Isotype Control

Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice After opening, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.
Use/Stability Stable for at least 1 year after receipt when stored at -20°C.
Documents
MSDS Download PDF
Product Specification Sheet
Datasheet Download PDF
Description

Apaf-1 is a cytoplasmic protein that forms one of the central hubs in the apoptosis regulatory network. It contains (from the N terminal) a caspase recruitment domain (CARD), an ATPase domain (NB-ARC), few short helical domains and then several copies of the WD40 repeat domain. Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome, mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis.

Product References
  1. Histone deacetylase activity regulates apaf-1 and caspase 3 expression in the developing mouse retina: D.M. Wallace, et al.; Invest. Ophthalmol. Vis. Sci. 47, 2765 (2006)
  2. Differential Apaf-1 levels allow cytochrome c to induce apoptosis in brain tumors but not in normal neural tissues: C.E. Johnson, et al.; PNAS 104, 20820 (2007)
  3. Chromatin modification of Apaf-1 restricts the apoptotic pathway in mature neurons: K.M. Wright, et al.; J. Cell Biol. 179, 825 (2007)
  4. Inhibition of apoptosome formation by suppression of Hsp90beta phosphorylation in tyrosine kinase-induced leukemias: M. Kurokawa, et al.; Mol. Cell Biol. 28, 5494 (2008)
  5. Suppression of the intrinsic apoptosis pathway by synaptic activity: F. Leveille, et al.; J. Neurosci. 30, 2623 (2010)
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