AdipoGen Life Sciences

anti-VEGFR-1 (human), mAb (EWC)

CHF 460.00
In stock
AG-20T-0106-C100100 µgCHF 460.00
More Information
Product Details
Synonyms Vascular Endothelial Growth Factor Receptor-1; FLT-1; Fms-like Tyrosine Kinase 1
Product Type Monoclonal Antibody
Properties
Clone EWC
Isotype Mouse IgG1
Immunogen/Antigen Recombinant human soluble VEGFR-1.
Application

ELISA: (1-10μg/ml)
Western Blot: (2-5μg/ml)
Optimal conditions must be determined individually for each application.

Crossreactivity Human
Specificity

The antibody will detect native human VEGFR-1/Flt-1 in ELISA experiments and on the surface of different human cell types.

Purity Protein G purified.
Purity Detail Protein G purified.
Formulation Lyophilized.
Reconstitution Centrifuge vial prior to opening. Reconstitute with sterile water to a concentration of 0.1-1.0mg/ml.
Isotype Negative Control

Mouse IgG1 Isotype Control

Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice After opening, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.
Use/Stability Stable for at least 6 months after receipt when stored at -20°C.
Documents
MSDS Download PDF
Product Specification Sheet
Datasheet Download PDF
Description

Recombinant human soluble vascular endothelial growth factor receptor-1 (sVEGFR-1) is the naturally occurring form and is a glycosylated monomeric protein. The biological function of sVEGFR-1 seems to be an endogenous regulator of angiogenesis, binding VEGF with the same affinity as the full-length receptor. VEGFR-1 is a tyrosine-protein kinase that acts as a cell-surface receptor for VEGFA, VEGFB and PGF, and plays an essential role in the development of embryonic vasculature, the regulation of angiogenesis, cell survival, cell migration, macrophage function, chemotaxis and cancer cell invasion. It may play an essential role as a negative regulator of embryonic angiogenesis by inhibiting excessive proliferation of endothelial cells. It can promote endothelial cell proliferation, survival and angiogenesis in adulthood. Its function in promoting cell proliferation seems to be cell-type specific. Promotes PGF-mediated proliferation of endothelial cells, proliferation of some types of cancer cells, but does not promote proliferation of normal fibroblasts (in vitro). It has a very high affinity for VEGFA and relatively low protein kinase activity. It may function as a negative regulator of VEGFA signaling by limiting the amount of free VEGFA and preventing its binding to KDR. Modulates KDR signaling by forming heterodimers with KDR. Ligand binding leads to the activation of several signaling cascades. Activation of phospholipase C-γ (PLCG) leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate and the activation of protein kinase C. Mediates phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, leading to activation of phosphatidylinositol kinase and the downstream signaling pathway. Mediates activation of MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. Phosphorylates SRC and YES1 and may also phosphorylate CBL.

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